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  • Spatiotemporally confined red light-controlled gene delivery at single-cell resolution using adeno-associated viral vectors.

Spatiotemporally confined red light-controlled gene delivery at single-cell resolution using adeno-associated viral vectors.

Science advances (2021-06-18)
Maximilian Hörner, Carolina Jerez-Longres, Anna Hudek, Sebastian Hook, O Sascha Yousefi, Wolfgang W A Schamel, Cindy Hörner, Matias D Zurbriggen, Haifeng Ye, Hanna J Wagner, Wilfried Weber
ABSTRACT

Methodologies for the controlled delivery of genetic information into target cells are of utmost importance for genetic engineering in both fundamental and applied research. However, available methods for efficient gene transfer into user-selected or even single cells suffer from low throughput, the need for complicated equipment, high invasiveness, or side effects by off-target viral uptake. Here, we engineer an adeno-associated viral (AAV) vector system that transfers genetic information into native target cells upon illumination with cell-compatible red light. This OptoAAV system allows adjustable and spatially resolved gene transfer down to single-cell resolution and is compatible with different cell lines and primary cells. Moreover, the sequential application of multiple OptoAAVs enables spatially resolved transduction with different transgenes. The approach presented is likely extendable to other classes of viral vectors and is expected to foster advances in basic and applied genetic research.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
重组人表皮细胞生长因子, EGF, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
Roche
cOmplete蛋白酶抑制剂Cocktail, Tablets provided in EASYpacks
Sigma-Aldrich
McCoy 5A培养基, Modified, with sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture