Merck
  • CDH18 is a fetal epicardial biomarker regulating differentiation towards vascular smooth muscle cells.

CDH18 is a fetal epicardial biomarker regulating differentiation towards vascular smooth muscle cells.

NPJ Regenerative medicine (2022-02-04)
Julia Junghof, Yuta Kogure, Tian Yu, Eva María Verdugo-Sivianes, Megumi Narita, Antonio Lucena-Cacace, Yoshinori Yoshida
摘要

The epicardium is a mesothelial layer covering the myocardium serving as a progenitor source during cardiac development. The epicardium reactivates upon cardiac injury supporting cardiac repair and regeneration. Fine-tuned balanced signaling regulates cell plasticity and cell-fate decisions of epicardial-derived cells (EPCDs) via epicardial-to-mesenchymal transition (EMT). However, powerful tools to investigate epicardial function, including markers with pivotal roles in developmental signaling, are still lacking. Here, we recapitulated epicardiogenesis using human induced pluripotent stem cells (hiPSCs) and identified type II classical cadherin CDH18 as a biomarker defining lineage specification in human active epicardium. The loss of CDH18 led to the onset of EMT and specific differentiation towards cardiac smooth muscle cells. Furthermore, GATA4 regulated epicardial CDH18 expression. These results highlight the importance of tracing CDH18 expression in hiPSC-derived epicardial cells, providing a model for investigating epicardial function in human development and disease and enabling new possibilities for regenerative medicine.

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单克隆抗 β-肌动蛋白抗体 小鼠抗, clone AC-15, ascites fluid
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MEC1 Mouse Embryonic Epicardial Cell Line, MEC1 mouse embryonic epicardial cell line is a useful model for cardiac developmental biology studies.