Merck
  • Development of a nanomaterial bio-screening platform for neurological applications.

Development of a nanomaterial bio-screening platform for neurological applications.

Nanomedicine : nanotechnology, biology, and medicine (2014-08-08)
Stuart Iain Jenkins, Paul Roach, Divya Maitreyi Chari
摘要

Nanoparticle platforms are being intensively investigated for neurological applications. Current biological models used to identify clinically relevant materials have major limitations, e.g. technical/ethical issues with live animal experimentation, failure to replicate neural cell diversity, limited control over cellular stoichiometries and poor reproducibility. High-throughput neuro-mimetic screening systems are required to address these challenges. We describe an advanced multicellular neural model comprising the major non-neuronal/glial cells of the central nervous system (CNS), shown to account for ~99.5% of CNS nanoparticle uptake. This model offers critical advantages for neuro-nanomaterials testing while reducing animal use: one primary source and culture medium for all cell types, standardized biomolecular corona formation and defined/reproducible cellular stoichiometry. Using dynamic time-lapse imaging, we demonstrate in real-time that microglia (neural immune cells) dramatically limit particle uptake in other neural subtypes (paralleling post-mortem observations after nanoparticle injection in vivo), highlighting the utility of the system in predicting neural handling of biomaterials.

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Sigma-Aldrich
抗生物素-FITC 抗体,小鼠单克隆抗体, clone BN-34, purified from hybridoma cell culture