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Role of the Plasmodium export element in trafficking parasite proteins to the infected erythrocyte.

Traffic (Copenhagen, Denmark) (2008-12-06)
Justin A Boddey, Robert L Moritz, Richard J Simpson, Alan F Cowman
ABSTRACT

The intracellular survival of Plasmodium falciparum within human erythrocytes is dependent on export of parasite proteins that remodel the host cell. Most exported proteins require a conserved motif (RxLxE/Q/D), termed the Plasmodium export element (PEXEL) or vacuolar targeting sequence (VTS), for targeting beyond the parasitophorous vacuole membrane and into the host cell; however, the precise role of this motif in export is poorly defined. We used transgenic P. falciparum expressing chimeric proteins to investigate the function of the PEXEL motif for export. The PEXEL constitutes a bifunctional export motif comprising a protease recognition sequence that is cleaved, in the endoplasmic reticulum, from proteins destined for export, in a PEXEL arginine- and leucine-dependent manner. Following processing, the remaining conserved PEXEL residue is required to direct the mature protein to the host cell. Furthermore, we demonstrate that N acetylation of proteins following N-terminal processing is a PEXEL-independent process that is insufficient for correct export to the host cell. This work defines the role of each residue in the PEXEL for export into the P. falciparum-infected erythrocyte.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
TWEEN ® 20, viscous liquid
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牛血清白蛋白 来源于牛血清, lyophilized powder, ≥96% (agarose gel electrophoresis)
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TWEEN ® 20, for molecular biology, viscous liquid
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TWEEN ® 20, BioXtra, viscous liquid
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牛血清白蛋白 来源于牛血清, fatty acid free, low endotoxin, lyophilized powder, BioReagent, suitable for cell culture, ≥96% (agarose gel electrophoresis)
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牛血清白蛋白 来源于牛血清, heat shock fraction, protease free, essentially globulin free, pH 7, ≥98%
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牛血清白蛋白 来源于牛血清, heat shock fraction, protease free, pH 7, ≥98%
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TWEEN ® 20, viscous liquid, suitable for cell culture
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牛血清白蛋白 来源于牛血清, lyophilized powder, essentially fatty acid free, ≥96% (agarose gel electrophoresis)
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牛血清白蛋白 来源于牛血清, heat shock fraction, lyophilized powder, essentially fatty acid free, ≥98% (agarose gel electrophoresis)
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牛血清白蛋白 来源于牛血清, lyophilized powder, essentially globulin free, ≥99% (agarose gel electrophoresis)
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牛血清白蛋白 来源于牛血清, heat shock fraction, pH 5.2, ≥96%
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牛血清白蛋白 来源于牛血清, powder, BioXtra
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