Merck
  • Verification and unmasking of widely used human esophageal adenocarcinoma cell lines.

Verification and unmasking of widely used human esophageal adenocarcinoma cell lines.

Journal of the National Cancer Institute (2010-01-16)
Jurjen J Boonstra, Ronald van Marion, David G Beer, Lin Lin, Paula Chaves, Catarina Ribeiro, A Dias Pereira, Lúcia Roque, S Jane Darnton, Nasser K Altorki, David S Schrump, David S Klimstra, Laura H Tang, James R Eshleman, Hector Alvarez, Yutaka Shimada, Herman van Dekken, Hugo W Tilanus, Winand N M Dinjens
摘要

For decades, hundreds of different human tumor type-specific cell lines have been used in experimental cancer research as models for their respective tumors. The veracity of experimental results for a specific tumor type relies on the correct derivation of the cell line. In a worldwide effort, we verified the authenticity of all available esophageal adenocarcinoma (EAC) cell lines. We proved that the frequently used cell lines SEG-1 and BIC-1 and the SK-GT-5 cell line are in fact cell lines from other tumor types. Experimental results based on these contaminated cell lines have led to ongoing clinical trials recruiting EAC patients, to more than 100 scientific publications, and to at least three National Institutes of Health cancer research grants and 11 US patents, which emphasizes the importance of our findings. Widespread use of contaminated cell lines threatens the development of treatment strategies for EAC.

材料
货号
品牌
产品描述

SK-GT-4 cell line human
FLO-1 cell line human
Sigma-Aldrich
KYAE-1 cell line
SK-GT-2 cell line
ESO51 cell line human
OACM5.1 C cell line human, 11012006
OE19 Cell Line human, 96071721
OE33 Cell Line human, Caucasian esophageal carcinoma, 96070808