The influence of collection solvents hexane and perfluorohexane on the permeation of the non-polar and non-volatile di-n-octyl disulfide (DOD) through nitrile glove material was investigated using the American Society for Testing and Materials (ASTM) F739-99a method. The weight and the thickness of the nitrile material increased about 6%, statistically significant at p<or=0.05, after 8 h of DOD permeation for both collection solvents. Infrared reflectance examination revealed no spectral changes to glove surfaces. Gas chromatography/mass spectrometry analysis determined that the apparent DOD steady state permeation rate was 903+/-285 microg/(cm2 h) for hexane collection, and 6.92+/-2.27 microg/(cm2 h) for perfluorohexane collection. The ASTM normalized breakthrough time for DOD was 7-30 min for hexane collection, and 30-75 min for perfluorohexane collection. Perfluorohexane is a promising collection solvent for non-polar and non-volatile compounds permeating nitrile material.