Circadian changes in long noncoding RNAs in the pineal gland.

Proceedings of the National Academy of Sciences of the United States of America (2012-08-07)
Steven L Coon, Peter J Munson, Praveen F Cherukuri, David Sugden, Martin F Rath, Morten Møller, Samuel J H Clokie, Cong Fu, Mary E Olanich, Zoila Rangel, Thomas Werner, James C Mullikin, David C Klein

Long noncoding RNAs (lncRNAs) play a broad range of biological roles, including regulation of expression of genes and chromosomes. Here, we present evidence that lncRNAs are involved in vertebrate circadian biology. Differential night/day expression of 112 lncRNAs (0.3 to >50 kb) occurs in the rat pineal gland, which is the source of melatonin, the hormone of the night. Approximately one-half of these changes reflect nocturnal increases. Studies of eight lncRNAs with 2- to >100-fold daily rhythms indicate that, in most cases, the change results from neural stimulation from the central circadian oscillator in the suprachiasmatic nucleus (doubling time = 0.5-1.3 h). Light exposure at night rapidly reverses (halving time = 9-32 min) levels of some of these lncRNAs. Organ culture studies indicate that expression of these lncRNAs is regulated by norepinephrine acting through cAMP. These findings point to a dynamic role of lncRNAs in the circadian system.


N6,2′-O-二丁酰基腺苷 3′,5′-环单磷酸 钠盐, ≥96% (HPLC), powder
N6,2′-O-二丁酰基腺苷 3′,5′-环单磷酸 钠盐, ≥97% (HPLC), powder