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Interaction of an amino-functionalized ionic liquid with enzymes: a fluorescence spectroscopy study.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy (2013-01-16)
Yunchang Fan, Sheli Zhang, Qiang Wang, Junhai Li, Haotian Fan, Dongkai Shan
ABSTRACT

The interaction of an amino-functionalized ionic liquid, 1-(2-aminoethyl)-3-butylimidazolium bromide ([NH(2)C(2)C(4)im]Br) with two enzymes, pepsin and papain was investigated using fluorescence spectroscopic technique. It is found that [NH(2)C(2)C(4)im]Br has strong ability to quench the intrinsic fluorescence of pepsin and papain. Quenching mechanisms are considered as static quenching for papain and dynamic quenching for pepsin, respectively. The binding constants and the number of binding sites (n) of [NH(2)C(2)C(4)im]Br to papain were calculated at different temperatures. The thermodynamic parameters such as free energy change (ΔG), enthalpy change (ΔH) and entropy change (ΔS), were calculated by thermodynamic equations. The values of ΔG, ΔH and ΔS suggest that interaction of [NH(2)C(2)C(4)im]Br with the two enzymes is spontaneous. Hydrogen bonding and van der Waals interactions play important roles in the binding process of [NH(2)C(2)C(4)im]Br to papain. However, hydrophobic interaction is the main driving force for the interaction of [NH(2)C(2)C(4)im]Br with pepsin. The results of three-dimensional fluorescence spectra show that [NH(2)C(2)C(4)im]Br has no obvious effects on the polypeptide structures of the two enzymes. Additionally, the [NH(2)C(2)C(4)im]Br-containing system can slightly increase the activities of the two enzymes.

MATERIALS
Product Number
Brand
Product Description

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