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[Role of NO/cGMP signaling cascade in the development of opium dependency].

Eksperimental'naia i klinicheskaia farmakologiia (2013-06-19)
D I Peregud, A A Iakovleva, M Iu Stepanichev, L F Panchenko, N V Guliaeva
ABSTRACT

This study was aimed at evaluating the role of nitric oxide (NO)/cyclic guanosine monophosphate (cGMP) cascade in mechanisms of morphine dependency formation. Morphine was introduced by intraperitoneal (i.p.) injections in rats twice per day over six days in doses increasing from 10 to 100 mg/kg, For evaluating the role of NO/cGMP cascade, NO synthase inhibitor L-N(G)-Nitroarginine methyl ester (L-NAME) was introduced (10 mg/kg, i.p.) 1 h prior to every injection of morphine. The L-NAME introduction led to enhancement of spontaneous withdrawal syndrome manifestations, which was accompanied by more pronounced decrease in the cGMP levels in midbrain and striatum. It is suggested that the region specific decrease in NO/cGMP cascade signaling activity in the brain can be among mechanisms determining the development of opium dependency.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Nω-硝基-L-精氨酸甲酯 盐酸盐, ≥98% (TLC), powder
Supelco
吗啡标准液 溶液, 1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®
Sigma-Aldrich
吗啡 硫酸盐 五水合物
Sigma-Aldrich
鸟苷 3',5'-环单磷酸, ≥98% (HPLC), powder
Supelco
吗啡 硫酸盐 溶液, 1.0 mg/mL in methanol, drug standard
Supelco
吗啡标准液 溶液, 100 μg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®