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  • Assay of beta-glucuronidase in bile following ion-pair extraction of pigments and bile acids.

Assay of beta-glucuronidase in bile following ion-pair extraction of pigments and bile acids.

Analytical biochemistry (1985-06-01)
W E Kurtin, W H Schwesinger
摘要

A method for improving the assay of beta-glucuronidase in hepatic and gallbladder bile is described. The method uses ion-pair extraction with N,N,N-triheptyl-1-heptanaminium bromide to remove pigments and bile acids. Conjugated bilirubin, unconjugated bilirubin, and taurine and glycine conjugates of deoxycholic and chenodeoxycholic acids are extracted efficiently from bile by the procedure. The sensitivity of the spectrophotometric assay of beta-glucuronidase in bile using phenolphthalein glucuronide is increased significantly.

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Sigma-Aldrich
苯酚 β-D-葡萄糖醛酸, β-glucuronidase substrate
Sigma-Aldrich
苯酚 β-D-葡萄糖醛酸 钠盐, β-glucuronidase substrate