• 主页
  • 查找结果
  • Lowry protein assay using an automatic microtiter plate spectrophotometer.

Lowry protein assay using an automatic microtiter plate spectrophotometer.

Analytical biochemistry (1986-03-01)
H J Fryer, G E Davis, M Manthorpe, S Varon
摘要

The method of protein determination reported by Lowry et al. (1951, J. Biol. Chem. 193, 265-275) has been adapted for use with 96-well microtiter plates and an automatic microplate spectrophotometer. The spectrophotometer has been interfaced with a computer which plots the standard curve and calculates the protein content of each sample. The adapted method offers advantages over previously reported methods in that it is more rapid and uses a smaller sample volume (100 microliters) for samples containing 3-300 micrograms/ml (0.3-30 micrograms/assay) of protein. The method of Bensadoun and Weinstein (1976, Anal. Biochem. 70, 241-252) for precipitating microgram amounts of protein away from substances which interfere with the Lowry assay has also been adapted to this microplate procedure. These techniques should be particularly useful for laboratories where large numbers of samples containing a wide range of protein concentrations are assayed.

材料
货号
品牌
产品描述

Sigma-Aldrich
Folin & Ciocalteu 酚试剂, suitable for determination of total protein by Lowry method, 2 N