• 主页
  • 查找结果
  • Mixed extracellular matrix ligands synergistically modulate integrin adhesion and signaling.

Mixed extracellular matrix ligands synergistically modulate integrin adhesion and signaling.

Journal of cellular physiology (2008-07-10)
Catherine D Reyes, Timothy A Petrie, Andrés J García
摘要

Cell adhesion to extracellular matrix (ECM) components through cell-surface integrin receptors is essential to the formation, maintenance and repair of numerous tissues, and therefore represents a central theme in the design of bioactive materials that successfully interface with the body. While the adhesive responses associated with a single ligand have been extensively analyzed, the effects of multiple integrin subtypes binding to multivalent ECM signals remain poorly understood. In the present study, we generated a high throughput platform of non-adhesive surfaces presenting well-defined, independent densities of two integrin-specific engineered ligands for the type I collagen (COL-I) receptor alpha(2)beta(1) and the fibronectin (FN) receptor alpha(5)beta(1) to evaluate the effects of integrin cross-talk on adhesive responses. Engineered surfaces displayed ligand density-dependent adhesive effects, and mixed ligand surfaces significantly enhanced cell adhesion strength and focal adhesion assembly compared to single FN and COL-I ligand surfaces. Moreover, surfaces presenting mixed COL-I/FN ligands synergistically enhanced FAK activation compared to the single ligand substrates. The enhanced adhesive activities of the mixed ligand surfaces also promoted elevated proliferation rates. Our results demonstrate interplay between multivalent ECM ligands in adhesive responses and downstream cellular signaling.

材料
货号
品牌
产品描述

Sigma-Aldrich
MAPTRIX-L-IKVAV, Laminin Mimetic, aqueous solution
Sigma-Aldrich
MAPTRIX-L-YIGSR, Laminin Mimetic, aqueous solution
Sigma-Aldrich
Anti-Integrin α5 Antibody, CT, Intracellular, serum, Chemicon®
Sigma-Aldrich
MAPTRIX-F-GRGDSP, Fibronectin Mimetic, aqueous solution
Sigma-Aldrich
MAPTRIX-L-KAFDITYVRLKF, Laminin Mimetic, aqueous solution
Sigma-Aldrich
MAPTRIX-V-FRHRNRKGY, Vitronectin Mimetic, aqueous solution
Sigma-Aldrich
MAPTRIX-F-RGD, Fibronectin Mimetic, aqueous solution
Sigma-Aldrich
MAPTrix Reagent, Low Molecular Weight, aqueous solution
Sigma-Aldrich
MAPTRIX-L-IKVAV, Laminin Mimetic, aqueous solution
Sigma-Aldrich
MAPTRIX-C-DGEA, Collagen Mimetic, aqueous solution
Sigma-Aldrich
Anti-Integrin α2 Antibody, serum, Chemicon®
Sigma-Aldrich
MAPTRIX-L-RKRLQVQLSIRT, Laminin Mimetic, aqueous solution
Sigma-Aldrich
MAPTrix Reagent, High Molecular Weight, aqueous solution
Sigma-Aldrich
MAPTRIX-C- GFPGER, Collagen Mimetic, aqueous solution
Sigma-Aldrich
MAPTRIX-F-RGD, Fibronectin Mimetic, aqueous solution
Sigma-Aldrich
MAPTRIX-F-REDV, Fibronectin Mimetic, aqueous solution
Sigma-Aldrich
MAPTRIX-M-VAEIDGIEL, Tenascin-C Mimetic, aqueous solution
Sigma-Aldrich
MAPTRIX-L-NRWHSIYITRFG, Laminin Mimetic, aqueous solution
Sigma-Aldrich
MAPTRIX-F-PHSRN, Fibronectin Mimetic, aqueous solution
Sigma-Aldrich
MAPTRIX-C-TAGSCLRKFSTM, Collagen Mimetic, aqueous solution
Sigma-Aldrich
MAPTRIX-L-TWYKIAFQRNRK, Laminin Mimetic, aqueous solution
Sigma-Aldrich
MAPTRIX-E-LRAHAVDING, Cadherin Mimetic, aqueous solution
Sigma-Aldrich
MAPTRIX-L-RYVVLPR, Laminin Mimetic, aqueous solution
Sigma-Aldrich
MAPTRIX-L-RQVFQVAYIIIKA, Laminin Mimetic, aqueous solution
Sigma-Aldrich
MAPTrix Reagent, Low Molecular Weight, aqueous solution
Sigma-Aldrich
MAPTrix Reagent, High Molecular Weight, aqueous solution
Sigma-Aldrich
MAPTrix Reagent, Low Molecular Weight, lyophilized powder
Sigma-Aldrich
MAPTrix Adhesive, Low Molecular Weight, Tyrosinase-Pretreated, lyophilized powder
Sigma-Aldrich
MAPTrix Adhesive, High Molecular Weight, Tyrosinase-Pretreated, lyophilized powder
Sigma-Aldrich
MAPTRIX-F- PHSRN, Fibronectin Mimetic, aqueous solution

社交媒体

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

Merck

科研、开发、生产。

作为生命科学行业的全球领先供应商,我们致力于为科研、生物技术开发和生产,以及制药药物疗法开发和生产提供各类解决方案和服务。

© 2021年版权归德国达姆施塔特默克集团(Merck KGaA)及/或其附属公司所有。版权所有。

未经许可,严禁复制本网站上的任何资料。