Quantification of endogenous aliphatic alcohols in serum and urine.

Journal of chromatography (1982-04-30)
H M Liebich, H J Buelow, R Kallmayer
摘要

The endogenous aliphatic alcohols ethanol, n-propanol, n-butanol, isobutanol and isopentanol in serum and urine were measured by gas chromatography-mass fragmentography. Whereas for the higher-molecular-weight alcohols extraction with dichloromethane is used, ethanol is determined by direct injection of serum and urine. When zero is assigned to all values below the detection limits of the procedures (0.1 mg/l for ethanol and 2 micrograms/l for each of the higher-molecular-weight alcohols) the following normal ranges are found: ethanol, 0-39 mg/l in serum and 0-46 mg per 24 h in urine; n-propanol, 0-48 micrograms/l in serum and 0-300 micrograms per 24 h in urine; n-butanol, 0-20 micrograms/l in serum and 0-18 micrograms per 24 h in urine. The isobutanol and isopentanol levels in the serum and urine of normal subjects are below the detection limit. For diabetic patients, on average increased levels are found for ethanol, n-propanol and n-butanol in serum and urine.

材料
货号
品牌
产品描述

Sigma-Aldrich
丙酮, ACS reagent, ≥99.5%
Sigma-Aldrich
甘油, for molecular biology, ≥99.0%
Sigma-Aldrich
丙酮, suitable for HPLC, ≥99.9%
Sigma-Aldrich
丙酮, HPLC Plus, for HPLC, GC, and residue analysis, ≥99.9%
Sigma-Aldrich
甘油, ≥99.5%
Sigma-Aldrich
甘油, ACS reagent, ≥99.5%
Sigma-Aldrich
丙酮, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., ≥99.5% (GC)
Sigma-Aldrich
甘油, ReagentPlus®, ≥99.0% (GC)
Sigma-Aldrich
正丁醇, anhydrous, 99.8%
Sigma-Aldrich
正丁醇, 99.9%
Sigma-Aldrich
1-丙醇, suitable for HPLC, ≥99.9%
Sigma-Aldrich
甘油, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for electrophoresis, ≥99% (GC)
Sigma-Aldrich
正丁醇, ACS reagent, ≥99.4%
Sigma-Aldrich
1-丙醇, anhydrous, 99.7%
Supelco
甘油, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
1-丙醇, ACS reagent, ≥99.5%
Sigma-Aldrich
丙酮, Laboratory Reagent, ≥99.5%
Sigma-Aldrich
正丁醇, for molecular biology, ≥99%
Sigma-Aldrich
正丁醇, suitable for HPLC, ≥99.7%
Sigma-Aldrich
甘油, meets USP testing specifications
Sigma-Aldrich
二甲胺 溶液, 40 wt. % in H2O
Sigma-Aldrich
甲基乙二醛 溶液, ~40% in H2O
Sigma-Aldrich
丙酮, suitable for HPLC, ≥99.8%
Sigma-Aldrich
甘油, BioXtra, ≥99% (GC)
Sigma-Aldrich
甘油 溶液, 83.5-89.5% (T)
Sigma-Aldrich
二甲胺, anhydrous, ≥99%
Supelco
丙酮, analytical standard
Sigma-Aldrich
二甲胺 溶液, 2.0 M in THF
Sigma-Aldrich
3-羟基丁酸, 95%
Supelco
正丁醇, analytical standard