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  • Separation, antitumor activities, and encapsulation of polypeptide from Chlorella pyrenoidosa.

Separation, antitumor activities, and encapsulation of polypeptide from Chlorella pyrenoidosa.

Biotechnology progress (2013-04-23)
Xiaoqin Wang, Xuewu Zhang
摘要

Chlorella pyrenoidosa is a unicellular green algae and has been a popular foodstuff worldwide. However, no reports on the antitumor peptides from such a microalgae are available in the literature. In this study, using low-temperature high-pressure extraction, enzymatic hydrolysis, ion exchange, and gel filtration chromatography, we separated a polypeptide that exhibited inhibitory activity on human liver cancer HepG2 cells, and named the polypeptide CPAP (C. pyrenoidosa antitumor polypeptide). Furthermore, the micro- and nanoencapsulation of CPAP were investigated by using two methods: complex coacervation and ionotropic gelation. The in vitro release tests revealed that CPAP was well preserved against gastric enzymatic degradation after micro/nanoencapsulation and the slowly controlled release in the intestine could be potentially achieved. These results suggest that CPAP may be a useful ingredient in food, nutraceutical, and pharmaceutical applications.

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Sigma-Aldrich
蛋白酶 来源于地衣芽孢杆菌, ≥2.4 U/g