Merck
  • FUS/TLS assembles into stress granules and is a prosurvival factor during hyperosmolar stress.

FUS/TLS assembles into stress granules and is a prosurvival factor during hyperosmolar stress.

Journal of cellular physiology (2013-04-30)
Reddy Ranjith K Sama, Catherine L Ward, Laura J Kaushansky, Nathan Lemay, Shinsuke Ishigaki, Fumihiko Urano, Daryl A Bosco
摘要

FUsed in Sarcoma/Translocated in LipoSarcoma (FUS/TLS or FUS) has been linked to several biological processes involving DNA and RNA processing, and has been associated with multiple diseases, including myxoid liposarcoma and amyotrophic lateral sclerosis (ALS). ALS-associated mutations cause FUS to associate with stalled translational complexes called stress granules under conditions of stress. However, little is known regarding the normal role of endogenous (non-disease linked) FUS in cellular stress response. Here, we demonstrate that endogenous FUS exerts a robust response to hyperosmolar stress induced by sorbitol. Hyperosmolar stress causes an immediate re-distribution of nuclear FUS to the cytoplasm, where it incorporates into stress granules. The redistribution of FUS to the cytoplasm is modulated by methyltransferase activity, whereas the inhibition of methyltransferase activity does not affect the incorporation of FUS into stress granules. The response to hyperosmolar stress is specific, since endogenous FUS does not redistribute to the cytoplasm in response to sodium arsenite, hydrogen peroxide, thapsigargin, or heat shock, all of which induce stress granule assembly. Intriguingly, cells with reduced expression of FUS exhibit a loss of cell viability in response to sorbitol, indicating a prosurvival role for endogenous FUS in the cellular response to hyperosmolar stress.

材料
货号
品牌
产品描述

Sigma-Aldrich
D -山梨醇, ≥98% (GC)
Supelco
山梨醇, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
D -山梨醇, 99% (GC)
Sigma-Aldrich
D -山梨醇, BioUltra, ≥99.0% (HPLC)
Sigma-Aldrich
D -山梨醇, ≥98% (GC), for molecular biology
Sigma-Aldrich
D -山梨醇, ≥98% (GC), BioReagent, suitable for cell culture, suitable for plant cell culture
Sigma-Aldrich
山梨醇 F 液 溶液, 70 wt. % in H2O, Contains mainly D-sorbitol with lesser amounts of other hydrogenated oligosaccharides
山梨醇, European Pharmacopoeia (EP) Reference Standard
Sigma-Aldrich
D -山梨醇, FCC, FG
Sigma-Aldrich
D -山梨醇, liquid, tested according to Ph. Eur.
Sigma-Aldrich
D -山梨醇, ≥98% (GC), BioXtra
Sigma-Aldrich
抗-二甲基精氨酸抗体,非对称性(ASYM24), serum, Upstate®