• 主页
  • 查找结果
  • In vitro gastrointestinal digestion of glabrous canaryseed proteins as affected by variety and thermal treatment.

In vitro gastrointestinal digestion of glabrous canaryseed proteins as affected by variety and thermal treatment.

Plant foods for human nutrition (Dordrecht, Netherlands) (2013-08-06)
Sahul H Rajamohamed, Alberta N A Aryee, Pierre Hucl, Carol Ann Patterson, Joyce I Boye
摘要

Glabrous or hairless canaryseed is a nutritional grain that could be a good addition to the diet if approved as a novel food. To assess the impact of thermal treatment on its digestibility; raw, roasted or boiled flours prepared from three different varieties of glabrous canaryseed were subjected to in vitro gastrointestinal digestion conditions and the effect on protein electrophoretic profiles was examined using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). Roasting was done by dry-heat in an oven at 176 °C for 12 min whereas boiling was done in water at 98 °C for 12 min. SDS-PAGE showed approximately twenty-five protein bands in the undigested raw flour with molecular masses (MM) ranging from <14 kDa to >97 kDa. The dominant proteins had low MM, between the ranges of ~57 to 12 kDa. Roasting markedly altered the protein electrophoretic profile with the appearance of large molecular weight aggregates. Canaryseed proteins were more easily digested after thermal treatment and under sequential gastric-duodenal conditions than under gastric or duodenal conditions alone. Furthermore, roasting appeared to have a greater impact on in vitro protein digestibility than boiling.

材料
货号
品牌
产品描述

Sigma-Aldrich
胃蛋白酶 来源于猪胃粘膜, powder, ≥250 units/mg solid
Sigma-Aldrich
胃蛋白酶 来源于猪胃粘膜, lyophilized powder, ≥3,200 units/mg protein
Sigma-Aldrich
α-糜蛋白酶 来源于牛胰腺, Type II, lyophilized powder, ≥40 units/mg protein
Sigma-Aldrich
胃蛋白酶 来源于猪胃粘膜, powder, ≥400 units/mg protein
胃蛋白酶, powder, European Pharmacopoeia (EP) Reference Standard
Sigma-Aldrich
胃蛋白酶 来源于猪胃粘膜, powder, slightly beige, ≥500 U/mg
Sigma-Aldrich
α-糜蛋白酶 来源于牛胰腺, (TLCK treated to inactivate residual tryspin activity), Type VII, essentially salt-free, lyophilized powder, ≥40 units/mg protein
Sigma-Aldrich
α-糜蛋白酶 来源于牛胰腺, Type I-S, essentially salt-free, lyophilized powder
Sigma-Aldrich
α-糜蛋白酶 来源于牛胰腺, suitable for protein sequencing, salt-free, lyophilized powder
Sigma-Aldrich
α-糜蛋白酶 来源于牛胰腺, ≥40 units/mg protein, vial of 5 mg
Sigma-Aldrich
胃蛋白酶 来源于猪胃粘膜, powder, slightly beige, 1200-2400 U/mg
Sigma-Aldrich
胃蛋白酶−琼脂糖 来源于猪胃粘膜, lyophilized powder, ≥30 units/mg dry solid
Sigma-Aldrich
胃蛋白酶 来源于猪胃粘膜, Suitable for manufacturing of diagnostic kits and reagents, lyophilized powder, ≥3200 units/mg protein
Sigma-Aldrich
α-糜蛋白酶 来源于人类胰腺, lyophilized powder
Sigma-Aldrich
α-Chymotrypsin−Agarose from bovine pancreas, lyophilized powder, 2,000-3,500 units/g agarose (One ml gel will yield 65-120 units)