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The function of the Na+-driven flagellum of Vibrio cholerae is determined by osmolality and pH.

Journal of bacteriology (2013-08-27)
Petra Halang, Sebastian Leptihn, Thomas Meier, Thomas Vorburger, Julia Steuber
ABSTRACT

Vibrio cholerae is motile by its polar flagellum, which is driven by a Na(+)-conducting motor. The stators of the motor, composed of four PomA and two PomB subunits, provide access for Na(+) to the torque-generating unit of the motor. To characterize the Na(+) pathway formed by the PomAB complex, we studied the influence of chloride salts (chaotropic, Na(+), and K(+)) and pH on the motility of V. cholerae. Motility decreased at elevated pH but increased if a chaotropic chloride salt was added, which rules out a direct Na(+) and H(+) competition in the process of binding to the conserved PomB D23 residue. Cells expressing the PomB S26A/T or D42N variants lost motility at low Na(+) concentrations but regained motility in the presence of 170 mM chloride. Both PomA and PomB were modified by N,N'-dicyclohexylcarbodiimide (DCCD), indicating the presence of protonated carboxyl groups in the hydrophobic regions of the two proteins. Na(+) did not protect PomA and PomB from this modification. Our study shows that both osmolality and pH have an influence on the function of the flagellum from V. cholerae. We propose that D23, S26, and D42 of PomB are part of an ion-conducting pathway formed by the PomAB stator complex.

MATERIALS
Product Number
Brand
Product Description

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N,N′-二环己基碳二亚胺, 99%
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N,N′-二环己基碳二亚胺 溶液, 1.0 M in methylene chloride
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二环己基碳二亚胺 溶液, 60 wt. % in xylenes