• 主页
  • 查找结果
  • Characterisation of different digestion susceptibility of lupin seed globulins.

Characterisation of different digestion susceptibility of lupin seed globulins.

Food chemistry (2013-09-24)
Jaroslaw Czubinski, Krzysztof Dwiecki, Aleksander Siger, Grazyna Neunert, Eleonora Lampart-Szczapa
摘要

This study describes in vitro digestion of lupin seed globulins by pancreatin, trypsin and chymotrypsin. Lupin seed globulins turned out to be almost totally susceptible to chymotrypsin digestion. When panceratin or trypsin were used for digestion of lupin seed globulins, γ-conglutin appeared to be resistant to proteolysis. Different fluorescence spectroscopic methods such as fluorescence anisotropy, fluorescence lifetimes and fluorescence quenching measurements were used for detailed characterisation of this phenomenon. A potential reason for γ-conglutin insensitivity to digestion may be related to the fact that lysine, as well as arginine, are positively charged at cell physiological pH. Simultaneously, flavonoids at this pH are partially ionised, which may lead to the occurrence of ionic interactions between these molecules at pH 7.5. The confirmation of this explanation may be the fact that γ-conglutin and vitexin form a static complex, which was observed using fluorescence quenching measurements.

材料
货号
品牌
产品描述

Sigma-Aldrich
胰脂肪酶 来源于猪胰腺, 8 × USP specifications
Sigma-Aldrich
胰脂肪酶 来源于猪胰腺, 4 × USP specifications
Sigma-Aldrich
α-糜蛋白酶 来源于牛胰腺, Type II, lyophilized powder, ≥40 units/mg protein
Sigma-Aldrich
胰脂肪酶 来源于猪胰腺, powder, suitable for cell culture, 4 × USP specifications
Sigma-Aldrich
胰脂肪酶 来源于猪胰腺, ≥3 × USP specifications
Sigma-Aldrich
α-糜蛋白酶 来源于牛胰腺, (TLCK treated to inactivate residual tryspin activity), Type VII, essentially salt-free, lyophilized powder, ≥40 units/mg protein
Sigma-Aldrich
α-糜蛋白酶 来源于牛胰腺, Type I-S, essentially salt-free, lyophilized powder
Sigma-Aldrich
α-糜蛋白酶 来源于牛胰腺, suitable for protein sequencing, salt-free, lyophilized powder
Sigma-Aldrich
α-糜蛋白酶 来源于牛胰腺, ≥40 units/mg protein, vial of 5 mg
Sigma-Aldrich
α-糜蛋白酶 来源于人类胰腺, lyophilized powder
Sigma-Aldrich
α-Chymotrypsin−Agarose from bovine pancreas, lyophilized powder, 2,000-3,500 units/g agarose (One ml gel will yield 65-120 units)