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  • Characterization of a novel chitinase, DkChi, from Dendrolimus kikuchii nucleopolyhedrovirus.

Characterization of a novel chitinase, DkChi, from Dendrolimus kikuchii nucleopolyhedrovirus.

Archives of virology (2013-07-06)
Qinghua Wang, Liangjian Qu, Zhilin Zhang, Yuzhu Wang, Yongan Zhang
ABSTRACT

Dendrolimus kikuchii Matsumura nucleopolyhedrovirus (DkNPV) is a novel nucleopolyhedrovirus strain that has exhibited high potential as biological control agent against D. kikuchii. In this work, a 1755-bp DkChi gene with sequence homology to a chitinase gene was cloned from the genomic DNA of DkNPV using a DNA fragment library. The DkChi gene, encoding 558 residues protein with a predicted mass of 61.6 kDa, was expressed at high levels in Escherichia coli and purified by affinity chromatography. We confirmed that the prepared protein was the DkChi protein by mass spectrometry analysis. Enzyme activity analysis showed that DkChi had both endo- and exo-chitinase activities. Interestingly, the DkChi protein displayed a strong insecticidal activity against Spodoptera exigua, Hyphantria cunea, Helicoverpa armigera and Lymantria dispar. The results suggest that DkChi is a good candidate protein for significantly contributing to pest control.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
灰链霉菌来源几丁质酶, lyophilized powder (essentially salt free), ≥200 units/g solid
Sigma-Aldrich
壳质酶 来源于绿色木霉, lyophilized powder, ≥600 units/g solid