Merck

Structural basis of Sec-independent membrane protein insertion by YidC.

Nature (2014-04-18)
Kaoru Kumazaki, Shinobu Chiba, Mizuki Takemoto, Arata Furukawa, Ken-ichi Nishiyama, Yasunori Sugano, Takaharu Mori, Naoshi Dohmae, Kunio Hirata, Yoshiko Nakada-Nakura, Andrés D Maturana, Yoshiki Tanaka, Hiroyuki Mori, Yuji Sugita, Fumio Arisaka, Koreaki Ito, Ryuichiro Ishitani, Tomoya Tsukazaki, Osamu Nureki
ABSTRACT

Newly synthesized membrane proteins must be accurately inserted into the membrane, folded and assembled for proper functioning. The protein YidC inserts its substrates into the membrane, thereby facilitating membrane protein assembly in bacteria; the homologous proteins Oxa1 and Alb3 have the same function in mitochondria and chloroplasts, respectively. In the bacterial cytoplasmic membrane, YidC functions as an independent insertase and a membrane chaperone in cooperation with the translocon SecYEG. Here we present the crystal structure of YidC from Bacillus halodurans, at 2.4 Å resolution. The structure reveals a novel fold, in which five conserved transmembrane helices form a positively charged hydrophilic groove that is open towards both the lipid bilayer and the cytoplasm but closed on the extracellular side. Structure-based in vivo analyses reveal that a conserved arginine residue in the groove is important for the insertion of membrane proteins by YidC. We propose an insertion mechanism for single-spanning membrane proteins, in which the hydrophilic environment generated by the groove recruits the extracellular regions of substrates into the low-dielectric environment of the membrane.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
L-精氨酸, reagent grade, ≥98%
Sigma-Aldrich
L-精氨酸, from non-animal source, meets EP, USP testing specifications, suitable for cell culture, 98.5-101.0%
SAFC
L-精氨酸
Sigma-Aldrich
L-精氨酸, 99%, FCC, FG
Supelco
L-精氨酸, Pharmaceutical Secondary Standard; Certified Reference Material
精氨酸, European Pharmacopoeia (EP) Reference Standard
Sigma-Aldrich
L-精氨酸, BioUltra, ≥99.5% (NT)