Electron tunneling in rhenium-modified Pseudomonas aeruginosa azurins.

Biochimica et biophysica acta (2004-04-22)
Jeremiah E Miller, Angel J Di Bilio, William A Wehbi, Michael T Green, A Katrine Museth, John R Richards, Jay R Winkler, Harry B Gray
摘要

Laser flash-quench methods have been used to generate tyrosine and tryptophan radicals in structurally characterized rhenium-modified Pseudomonas aeruginosa azurins. Cu(I) to "Re(II)" electron tunneling in Re(H107) azurin occurs in the microsecond range. This reaction is much faster than that studied previously for Cu(I) to Ru(III) tunneling in Ru(H107) azurin, suggesting that a multistep ("hopping") mechanism might be involved. Although a Y108 radical can be generated by flash-quenching a Re(H107)M(II) (M=Cu, Zn) protein, the evidence suggests that it is not an active intermediate in the enhanced Cu(I) oxidation. Rather, the likely explanation is rapid conversion of Re(II)(H107) to deprotonated Re(I)(H107 radical), followed by electron tunneling from Cu(I) to the hole in the imidazole ligand.

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铼, powder, 99.995% trace metals basis
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铼, foil, thickness 0.25 mm, 99.98% trace metals basis
Sigma-Aldrich
铼, powder, −100 mesh, ≥99.9% trace metals basis
铼, wire reel, 0.025m, diameter 1.0mm, annealed, 99.97%
铼, wire reel, 0.05m, diameter 0.5mm, annealed, 99.97%
铼, wire reel, 0.05m, diameter 1.0mm, annealed, 99.97%
铼, wire reel, 0.1m, diameter 0.076mm, annealed, 99.97%
铼, wire reel, 0.1m, diameter 0.10mm, annealed, 99.97%
铼, wire reel, 0.1m, diameter 0.125mm, annealed, 99.97%
铼, wire reel, 0.1m, diameter 0.15mm, annealed, 99.97%
铼, wire reel, 0.1m, diameter 0.178mm, annealed, 99.97%
铼, wire reel, 0.1m, diameter 0.25mm, annealed, 99.97%
铼, wire reel, 0.1m, diameter 0.2mm, annealed, 99.97%
铼, wire reel, 0.1m, diameter 0.3mm, annealed, 99.97%
铼, wire reel, 0.1m, diameter 0.5mm, annealed, 99.97%
铼, wire reel, 0.1m, diameter 1.0mm, annealed, 99.97%
铼, wire reel, 0.2m, diameter 0.076mm, annealed, 99.97%
铼, wire reel, 0.2m, diameter 0.10mm, annealed, 99.97%
铼, wire reel, 0.2m, diameter 0.125mm, annealed, 99.97%
铼, wire reel, 0.2m, diameter 0.15mm, annealed, 99.97%
铼, wire reel, 0.2m, diameter 0.178mm, annealed, 99.97%
铼, wire reel, 0.2m, diameter 0.25mm, annealed, 99.97%
铼, wire reel, 0.2m, diameter 0.2mm, annealed, 99.97%
铼, wire reel, 0.2m, diameter 0.3mm, annealed, 99.97%
铼, wire reel, 0.2m, diameter 0.5mm, annealed, 99.97%
铼, wire reel, 0.2m, diameter 1.0mm, annealed, 99.97%
铼, wire reel, 0.5m, diameter 0.076mm, annealed, 99.97%
铼, wire reel, 0.5m, diameter 0.10mm, annealed, 99.97%
铼, wire reel, 0.5m, diameter 0.125mm, annealed, 99.97%
铼, wire reel, 0.5m, diameter 0.15mm, annealed, 99.97%