Cell uptake and oral absorption of titanium dioxide nanoparticles.

Toxicology letters (2014-05-06)
G Janer, E Mas del Molino, E Fernández-Rosas, A Fernández, S Vázquez-Campos
摘要

Large efforts are invested on the development of in vitro tests to evaluate nanomaterial (NM) toxicity. In order to assess the relevance of the adverse effects identified in in vitro toxicity tests a thorough understanding of the biokinetics of NMs is critical. We used different in vitro and in vivo test methods to evaluate cell uptake and oral absorption of titanium dioxide nanoparticles (TiO2 NPs). These NPs were readily uptaken by A549 cells (carcinomic human alveolar basal epithelial cells) in vitro. Such rapid uptake contrasted with a very low oral absorption in a differentiated Caco-2 monolayer system (human epithelial colorectal adenocarcinoma cells) and after oral gavage administration to rats. In this oral study, no significant increase in the levels of titanium was recorded by ICP-MS in any of the tissues evaluated (including among other: small intestine, Peyer's patches, mesenteric lymph nodes, liver, and spleen). No NPs were observed by TEM in sections of the small intestine, except for several particles in the cytoplasm of a cell from a Peyer's Patch area. The observation of NPs in Peyer's Patch suggests that the Caco-2 monolayer system is likely to underestimate the potential for oral absorption of NPs and that the model could be improved by including M-cells in co-culture.

材料
货号
品牌
产品描述

Sigma-Aldrich
二氧化钛(IV), nanopowder, 21 nm primary particle size (TEM), ≥99.5% trace metals basis
Sigma-Aldrich
氧化钛 (IV),锐钛矿, nanopowder, <25 nm particle size, 99.7% trace metals basis
Sigma-Aldrich
二氧化钛(IV), ReagentPlus®, ≥99%
Sigma-Aldrich
氧化钛 (IV),锐钛矿, powder, 99.8% trace metals basis
Sigma-Aldrich
钛 (IV) 氧化物,金红石型, powder, <5 μm, ≥99.9% trace metals basis
Sigma-Aldrich
氧化钛 (IV),锐钛矿, powder, −325 mesh, ≥99% trace metals basis
Sigma-Aldrich
氧化钛(IV),金红石和锐钛矿混合物, nanopowder, <100 nm particle size (BET), 99.5% trace metals basis
Sigma-Aldrich
钛 (IV) 氧化物,金红石型, nanopowder, <100 nm particle size, 99.5% trace metals basis
Sigma-Aldrich
二氧化钛(IV), puriss., meets analytical specification of Ph. Eur., BP, USP, 99-100.5%
Sigma-Aldrich
钛, foil, thickness 0.127 mm, 99.7% trace metals basis
Sigma-Aldrich
钛, powder, <45 μm avg. part. size, 99.98% trace metals basis
Sigma-Aldrich
钛, foil, thickness 0.25 mm, 99.7% trace metals basis
Sigma-Aldrich
钛, powder, −100 mesh, 99.7% trace metals basis
Sigma-Aldrich
氧化钛(IV),金红石和锐钛矿混合物, nanoparticles, <150 nm particle size (volume distribution, DLS), dispersion, 40 wt. % in H2O, 99.5% trace metals basis
Sigma-Aldrich
钛 (IV) 氧化物,金红石型, ≥99.98% trace metals basis
Sigma-Aldrich
钛 (IV) 氧化物,金红石型, 99.995% trace metals basis
Sigma-Aldrich
钛, foil, thickness 0.025 mm, 99.98% trace metals basis
Sigma-Aldrich
钛, sponge, 3-19 mm, 99.5% trace metals basis
Sigma-Aldrich
钛, wire, diam. 0.25 mm, 99.7% trace metals basis
Sigma-Aldrich
钛, nanoparticles, dispersion, <100 nm particle size, in mineral oil, 98.5% trace metals basis
Sigma-Aldrich
二氧化钛(IV), contains 1% Mn as dopant, nanopowder, <100 nm particle size (BET), ≥97%
Sigma-Aldrich
二氧化钛(IV), nanowires, diam. × L ~10 nm × 10 μm
Sigma-Aldrich
钛, sputtering target, diam. × thickness 2.00 in. × 0.25 in., 99.995% trace metals basis
Sigma-Aldrich
钛, foil, thickness 0.5 mm, 99.99% trace metals basis
Sigma-Aldrich
氧化钛(IV),金红石和锐钛矿混合物, nanoparticle, <250 nm particle size (DLS), paste, 53-57 wt. % in diethylene glycol monobutyl ether/ethylene glycol, 99.9% trace metals basis
Sigma-Aldrich
钛, wire, diam. 0.81 mm, 99.7% trace metals basis
Sigma-Aldrich
钛, foil, thickness 0.25 mm, 99.99% trace metals basis
Sigma-Aldrich
钛, foil, thickness 2.0 mm, 99.7% trace metals basis
Sigma-Aldrich
二氧化钛(IV), nanowires, diam. × L ~100 nm × 10 μm
Sigma-Aldrich
钛, wire, diam. 1.0 mm, 99.99% trace metals basis