Merck
  • Home
  • Search Results
  • Arylsulfatase A bound to poly(butyl cyanoacrylate) nanoparticles for enzyme replacement therapy--physicochemical evaluation.

Arylsulfatase A bound to poly(butyl cyanoacrylate) nanoparticles for enzyme replacement therapy--physicochemical evaluation.

Die Pharmazie (2014-07-31)
A Mühlstein, S Gelperina, E Shipulo, O Maksimenko, J Kreuter
ABSTRACT

Arylsulfatase A (ASA) deficiency is the cause of metachromatic leucodystrophy (MLD), a lysosomal storage disease associated with severe neurological disorders. Poly(butyl cyanoacrylate) (PBCA) nanoparticles overcoated with polysorbate 80 enabled the delivery of several drugs across the blood-brain barrier to the brain suggesting that these nanoparticles also may transport ASA across this barrier. The objective of this research, therefore, was to evaluate the feasibility of loading ASA onto PBCA nanoparticles. A stable ASA-loaded PBCA nanoparticle formulation was developed that could be easily freeze-dried and stored over a period of more than 8 weeks. The maximum loading capacity for this enzyme was -59 microg per 1 mg of PBCA. In the presence of 3% sucrose as a lyoprotector the activity of freeze-dried ASA was found to be 100% recoverable.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
氢氧化钠, reagent grade, ≥98%, pellets (anhydrous)
Sigma-Aldrich
蔗糖, for molecular biology, ≥99.5% (GC)
Sigma-Aldrich
氢氧化钠 溶液, BioUltra, for molecular biology, 10 M in H2O
Sigma-Aldrich
氢氧化钠, ACS reagent, ≥97.0%, pellets
Sigma-Aldrich
蔗糖, ≥99.5% (GC)
Sigma-Aldrich
氢氧化钠 溶液, 50% in H2O
Sigma-Aldrich
蔗糖, BioXtra, ≥99.5% (GC)
Sigma-Aldrich
D -甘露醇, ≥98%
Sigma-Aldrich
蔗糖, BioUltra, for molecular biology, ≥99.5% (HPLC)
Sigma-Aldrich
氢氧化钠, BioXtra, ≥98% (acidimetric), pellets (anhydrous)
Sigma-Aldrich
氢氧化钠 溶液, 1.0 N, BioReagent, suitable for cell culture
Supelco
蔗糖, Pharmaceutical Secondary Standard; Certified Reference Material
Supelco
甘露醇, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
蔗糖, puriss., meets analytical specification of Ph. Eur., BP, NF
Sigma-Aldrich
蔗糖, ACS reagent
Sigma-Aldrich
氢氧化钠, puriss., meets analytical specification of Ph. Eur., BP, NF, E524, 98-100.5%, pellets
USP
蔗糖, United States Pharmacopeia (USP) Reference Standard
Sigma-Aldrich
D -甘露醇, ACS reagent
Sigma-Aldrich
蔗糖, ≥99.5%
Sigma-Aldrich
蔗糖, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥99.5% (GC)
Supelco
氢氧化钠溶液, 0.1 M NaOH in water (0.1N), Eluent concentrate for IC
Supelco
海藻糖, Pharmaceutical Secondary Standard; Certified Reference Material
Supelco
氢氧化钠 溶液, 49-51% in water, eluent for IC
Millipore
蔗糖, ACS reagent, suitable for microbiology, ≥99.0%
Sigma-Aldrich
氢氧化钠 溶液, 5.0 M
Sigma-Aldrich
D-乳糖 一水合物, BioUltra, ≥99.5% (HPLC)
Sigma-Aldrich
氢氧化钠, reagent grade, 97%, powder
Sigma-Aldrich
D -甘露醇, meets EP, FCC, USP testing specifications
Supelco
乳糖一水合物, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
氢氧化钠, puriss. p.a., ACS reagent, reag. Ph. Eur., K ≤0.02%, ≥98%, pellets