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  • Acinetobacter species in the skin microbiota protect against allergic sensitization and inflammation.

Acinetobacter species in the skin microbiota protect against allergic sensitization and inflammation.

The Journal of allergy and clinical immunology (2014-09-30)
Nanna Fyhrquist, Lasse Ruokolainen, Alina Suomalainen, Sari Lehtimäki, Ville Veckman, Johanna Vendelin, Piia Karisola, Maili Lehto, Terhi Savinko, Hanna Jarva, Timo U Kosunen, Jukka Corander, Petri Auvinen, Lars Paulin, Leena von Hertzen, Tiina Laatikainen, Mika Mäkelä, Tari Haahtela, Dario Greco, Ilkka Hanski, Harri Alenius
摘要

The human commensal microbiota interacts in a complex manner with the immune system, and the outcome of these interactions might depend on the immune status of the subject. Previous studies have suggested a strong allergy-protective effect for Gammaproteobacteria. Here we analyze the skin microbiota, allergic sensitization (atopy), and immune function in a cohort of adolescents, as well as the influence of Acinetobacter species on immune responses in vitro and in vivo. The skin microbiota of the study subjects was identified by using 16S rRNA sequencing. PBMCs were analyzed for baseline and allergen-stimulated mRNA expression. In in vitro assays human monocyte-derived dendritic cells and primary keratinocytes were incubated with Acinetobacter lwoffii. Finally, in in vivo experiments mice were injected intradermally with A lwoffii during the sensitization phase of the asthma protocol, followed by readout of inflammatory parameters. In healthy subjects, but not in atopic ones, the relative abundance of Acinetobacter species was associated with the expression of anti-inflammatory molecules by PBMCs. Moreover, healthy subjects exhibited a robust balance between anti-inflammatory and TH1/TH2 gene expression, which was related to the composition of the skin microbiota. In cell assays and in a mouse model, Acinetobacter species induced strong TH1 and anti-inflammatory responses by immune cells and skin cells and protected against allergic sensitization and lung inflammation through the skin. These results support the hypothesis that skin commensals play an important role in tuning the balance of TH1, TH2, and anti-inflammatory responses to environmental allergens.

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Sigma-Aldrich
白蛋白 来源于鸡蛋白, lyophilized powder, ≥98% (agarose gel electrophoresis)
Sigma-Aldrich
白蛋白 来源于鸡蛋白, lyophilized powder, ≥98% (agarose gel electrophoresis)
Sigma-Aldrich
白蛋白 来源于鸡蛋白, powder, 62-88% (agarose gel electrophoresis)
Sigma-Aldrich
白蛋白 来源于鸡蛋白, lyophilized powder, ≥90% (agarose gel electrophoresis)
Sigma-Aldrich
白蛋白 来源于鸡蛋白, lyophilized powder
Supelco
白蛋白 来源于鸡蛋白, For use as a marker in SDS-PAGE