• 主页
  • 查找结果
  • Metabolism and mis-metabolism of the neuropathological signature protein TDP-43.

Metabolism and mis-metabolism of the neuropathological signature protein TDP-43.

Journal of cell science (2014-05-27)
Chi-Chen Huang, Jayarama Krishnan Bose, Pritha Majumder, Kuen-Haur Lee, Jen-Tse Joseph Huang, Jeffrey K Huang, Che-Kun James Shen
摘要

TDP-43 (also known as TARDBP) is a pathological signature protein of neurodegenerative diseases, with TDP-43 proteinopathies including frontotemporal lobar degeneration (FTLD)-TDP and amyotrophic lateral sclerosis (ALS)-TDP. These TDP-43 proteinopathies are characterized by cytoplasmic insoluble TDP-43-positive aggregates in the diseased cells, the formation of which requires the seeding of TDP-25 fragment generated by caspase cleavage of TDP-43. We have investigated the metabolism and mis-metabolism of TDP-43 in cultured cells and found that endogenous and exogenously overexpressed TDP-43 is degraded not only by the ubiquitin proteasome system (UPS) and macroautophagy, but also by the chaperone-mediated autophagy (CMA) mediated through an interaction between Hsc70 (also known as HSPA8) and ubiquitylated TDP-43. Furthermore, proteolytic cleavage of TDP-43 by caspase(s) is a necessary intermediate step for degradation of the majority of the TDP-43 protein, with the TDP-25 and TDP-35 fragments being the main substrates. Finally, we have determined the threshold level of the TDP-25 fragment that is necessary for formation of the cytosolic TDP-43-positive aggregates in cells containing the full-length TDP-43 at an elevated level close to that found in patients with TDP-43 proteinopathies. A comprehensive model of the metabolism and mis-metabolism of TDP-43 in relation to these findings is presented.

材料
货号
品牌
产品描述

Sigma-Aldrich
DAPI, for nucleic acid staining
Sigma-Aldrich
氯化铵, for molecular biology, suitable for cell culture, ≥99.5%
Sigma-Aldrich
放线菌酮, from microbial, ≥94% (TLC)
Sigma-Aldrich
环己酰亚胺,大包装, ≥95% (HPLC)
Sigma-Aldrich
放线菌酮 溶液, Ready-Made Solution, microbial, 100 mg/mL in DMSO, 0.2 μm filtered
Sigma-Aldrich
3-甲基腺嘌呤, autophagy inhibitor
Sigma-Aldrich
氯化铵, 99.998% trace metals basis
Sigma-Aldrich
氯化铵, ReagentPlus®, ≥99.5%
Sigma-Aldrich
Z-Leu-Leu-Leu-al, ≥90% (HPLC)
Supelco
放线菌酮, PESTANAL®, analytical standard
Sigma-Aldrich
抗LC3 兔抗, ~1 mg/mL, affinity isolated antibody, buffered aqueous solution
Millipore
放线菌酮 溶液, 0.1%, suitable for microbiology
Sigma-Aldrich
放线菌酮, Biotechnology Performance Certified
Supelco
氯化铵, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
氯化铵, BioUltra, for molecular biology, ≥99.5% (AT)
Sigma-Aldrich
氯化铵, 99.99% trace metals basis
Supelco
铵离子标准溶液, 1000 mg/kg N, analytical standard (for ion-selective electrodes)
Sigma-Aldrich
氯化铵, puriss., meets analytical specification of Ph. Eur., BP, USP, FCC, 99.5-100.5% (calc. to the dried substance)
Sigma-Aldrich
氯化铵, tested according to Ph. Eur.
Sigma-Aldrich
Anti-ANXA3 antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Sigma-Aldrich
Anti-MAP1LC3A antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Sigma-Aldrich
ANTI-LC3 (APG8A) antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution
Sigma-Aldrich
Anti-MAP1LC3A antibody produced in rabbit, purified immunoglobulin, buffered aqueous solution
Sigma-Aldrich
Anti-NUP62 antibody produced in rabbit, purified immunoglobulin, buffered aqueous solution
Sigma-Aldrich
Anti-MAP1LC3A antibody produced in rabbit, affinity isolated antibody
Sigma-Aldrich
Anti-MAP1LC3A antibody produced in rabbit, affinity isolated antibody
Sigma-Aldrich
Anti-TARDBP (ab1) antibody produced in rabbit, affinity isolated antibody
Sigma-Aldrich
Anti-TDP-43 antibody produced in rabbit, ~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution
Sigma-Aldrich
Anti-ANXA3 (AB2) antibody produced in rabbit, IgG fraction of antiserum
Sigma-Aldrich
Anti-ANXA3 (AB1) antibody produced in rabbit, IgG fraction of antiserum

社交媒体

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

Merck

科研、开发、生产。

作为生命科学行业的全球领先供应商,我们致力于为科研、生物技术开发和生产,以及制药药物疗法开发和生产提供各类解决方案和服务。

© 2021年版权归德国达姆施塔特默克集团(Merck KGaA)及/或其附属公司所有。版权所有。

未经许可,严禁复制本网站上的任何资料。