Merck
  • Home
  • Search Results
  • Adolescent exposure to THC in female rats disrupts developmental changes in the prefrontal cortex.

Adolescent exposure to THC in female rats disrupts developmental changes in the prefrontal cortex.

Neurobiology of disease (2014-10-05)
Tiziana Rubino, Pamela Prini, Fabiana Piscitelli, Erica Zamberletti, Massimo Trusel, Miriam Melis, Claudia Sagheddu, Alessia Ligresti, Raffaella Tonini, Vincenzo Di Marzo, Daniela Parolaro
ABSTRACT

Current concepts suggest that exposure to THC during adolescence may act as a risk factor for the development of psychiatric disorders later in life. However, the molecular underpinnings of this vulnerability are still poorly understood. To analyze this, we investigated whether and how THC exposure in female rats interferes with different maturational events occurring in the prefrontal cortex during adolescence through biochemical, pharmacological and electrophysiological means. We found that the endocannabinoid system undergoes maturational processes during adolescence and that THC exposure disrupts them, leading to impairment of both endocannabinoid signaling and endocannabinoid-mediated LTD in the adult prefrontal cortex. THC also altered the maturational fluctuations of NMDA subunits, leading to larger amounts of gluN2B at adulthood. Adult animals exposed to THC during adolescence also showed increased AMPA gluA1 with no changes in gluA2 subunits. Finally, adolescent THC exposure altered cognition at adulthood. All these effects seem to be triggered by the disruption of the physiological role played by the endocannabinoid system during adolescence. Indeed, blockade of CB1 receptors from early to late adolescence seems to prevent the occurrence of pruning at glutamatergic synapses. These results suggest that vulnerability of adolescent female rats to long-lasting THC adverse effects might partly reside in disruption of the pivotal role played by the endocannabinoid system in the prefrontal cortex maturation.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
二甲基亚砜, Hybri-Max, sterile-filtered, BioReagent, suitable for hybridoma, ≥99.7%
Sigma-Aldrich
二甲基亚砜, anhydrous, ≥99.9%
Sigma-Aldrich
二甲基亚砜, for molecular biology
Sigma-Aldrich
二甲基亚砜, ACS reagent, ≥99.9%
Sigma-Aldrich
二甲基亚砜, sterile-filtered, BioPerformance Certified, meets EP, USP testing specifications, suitable for hybridoma
Sigma-Aldrich
二乙醚, contains 1 ppm BHT as inhibitor, anhydrous, ≥99.7%
Sigma-Aldrich
二甲基亚砜, suitable for HPLC, ≥99.7%
Sigma-Aldrich
二甲基亚砜, ReagentPlus®, ≥99.5%
Sigma-Aldrich
二甲基亚砜, ≥99.5% (GC), suitable for plant cell culture
Sigma-Aldrich
二乙醚, anhydrous, ACS reagent, ≥99.0%, contains BHT as inhibitor
Sigma-Aldrich
二乙醚, suitable for HPLC, ≥99.9%, inhibitor-free
Sigma-Aldrich
磷酸钠, 96%
Sigma-Aldrich
二乙醚, ACS reagent, anhydrous, ≥99.0%, contains BHT as inhibitor
Sigma-Aldrich
二甲基亚砜, meets EP testing specifications, meets USP testing specifications
Sigma-Aldrich
二甲基亚砜, puriss. p.a., ACS reagent, ≥99.9% (GC)
Sigma-Aldrich
二乙醚, contains BHT as inhibitor, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., ≥99.8%
Sigma-Aldrich
二甲基亚砜, BioUltra, for molecular biology, ≥99.5% (GC)
Sigma-Aldrich
二甲基亚砜, PCR Reagent
Sigma-Aldrich
二甲基亚砜 溶液, 50 wt. % in H2O
Sigma-Aldrich
二乙醚, ACS reagent, ≥98.0%, contains ≤2% ethanol and ≤10ppm BHT as inhibitor
Supelco
二乙醚, analytical standard
Supelco
二甲基亚砜, analytical standard
Supelco
二甲基亚砜, for inorganic trace analysis, ≥99.99995% (metals basis)
Sigma-Aldrich
二乙醚, reagent grade, ≥98%, contains ≤2% ethanol and ≤10ppm BHT as inhibitor
USP
二甲基亚砜, United States Pharmacopeia (USP) Reference Standard
Sigma-Aldrich
AM251, >98% (HPLC), solid
二甲基亚砜, European Pharmacopoeia (EP) Reference Standard
Sigma-Aldrich
二乙醚, puriss., contains ~5 mg/L 2,6-di-tert.-butyl-4-methylphenol as stabilizer, meets analytical specification of Ph. Eur., BP, ≥99.5% (GC)
Sigma-Aldrich
二甲基亚砜, puriss. p.a., dried, ≤0.02% water
Sigma-Aldrich
8-Octanoyloxypyrene-1,3,6-trisulfonic acid trisodium salt, suitable for fluorescence, ≥90% (HPCE)