Merck
  • 主页
  • 查找结果
  • Schistosome infections induce significant changes in the host biliary proteome.

Schistosome infections induce significant changes in the host biliary proteome.

Journal of proteomics (2014-12-03)
Eduardo de la Torre-Escudero, Ricardo Pérez-Sánchez, Raúl Manzano-Román, Ana Oleaga
摘要

Schistosomiasis is a disease caused by blood trematodes affecting man and animals that represents an important human health and veterinary problem. Main damages caused by this infection are a consequence of the host inflammatory reaction against the parasite eggs trapped inside the liver. Despite that the hepatic pathology of schistosomiasis is very well known, there are no specific studies dealing with the schistosome infection effects on the biliary function. The purpose of this work was to analyse the changes induced by Schistosoma bovis infection in the biliary proteome. For this, whole gallbladders from S. bovis-infected and non-infected mice were dissected, homogenized and fractionated by differential centrifugation. The resulting protein fractions were resolved by SDS-PAGE, the gels were sliced, and the gel pieces analysed by LC-MS/MS. Altogether, we identified 1937 proteins, which were classified according to their "protein class" and "molecular function", and then subjected to an "Enrichment analysis". The differences found in gallbladder proteomes between S. bovis-infected and non-infected mice are analysed. We show that chronic schistosome infections cause significant changes in the biliary proteome that may produce physiological alterations and affect the therapeutic actions of drugs when administered to human patients and animals with schistosomiasis. To identify the changes induced by the schistosome infection in bile protein composition, and therefore in bile function, we compared the proteome of the gallbladders collected from non-infected healthy mice and from mice infected with S. bovis during 4months. For this, gallbladders from both groups of mice were homogenized and these homogenates were fractionated by serial centrifugation and acrylamide gel electrophoresis. The proteins were in gel digested and analysed by LC-MS/MS for identification. The present work reports the first data on the proteome of the mouse gallbladder and provides a comprehensive catalogue of biliary proteins that may be of great use in other studies addressing biliary physiology and pathology. We observed that there were significant differences in the biliary proteome of mice infected and non-infected with S. bovis and that chronic schistosome infections may produce important physiological alterations in the bile function.

材料
货号
品牌
产品描述

Sigma-Aldrich
乙腈, suitable for HPLC, gradient grade, ≥99.9%
Sigma-Aldrich
甲醇, suitable for HPLC, ≥99.9%
Sigma-Aldrich
乙腈, anhydrous, 99.8%
Sigma-Aldrich
甲醇, anhydrous, 99.8%
Sigma-Aldrich
三氟乙酸, suitable for HPLC, ≥99.0%
Sigma-Aldrich
三氟乙酸, ReagentPlus®, 99%
Sigma-Aldrich
乙酸, glacial, ReagentPlus®, ≥99%
Sigma-Aldrich
乙腈, HPLC Plus, ≥99.9%
Sigma-Aldrich
甲醇, ACS reagent, ≥99.8%
Sigma-Aldrich
乙酸, glacial, ACS reagent, ≥99.7%
Sigma-Aldrich
L -还原型谷胱甘肽, ≥98.0%
Sigma-Aldrich
甲醇, HPLC Plus, ≥99.9%
Sigma-Aldrich
甲醇, suitable for HPLC, gradient grade, ≥99.9%
Sigma-Aldrich
三氟乙酸, puriss. p.a., suitable for HPLC, ≥99.0% (GC)
Sigma-Aldrich
乙腈, ACS reagent, ≥99.5%
Sigma-Aldrich
乙酸, glacial, puriss., meets analytical specification of Ph. Eur., BP, USP, FCC, 99.8-100.5%
Sigma-Aldrich
乙腈, suitable for HPLC, gradient grade, ≥99.9%
Sigma-Aldrich
甲醇, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., ≥99.8% (GC)
Sigma-Aldrich
L -还原型谷胱甘肽, suitable for cell culture, BioReagent, ≥98.0%, powder
Sigma-Aldrich
乙酸, glacial, ≥99.99% trace metals basis
Supelco
甲醇, analytical standard
Sigma-Aldrich
乙酸 溶液, suitable for HPLC
Sigma-Aldrich
乙腈 溶液, contains 0.1 % (v/v) trifluoroacetic acid, suitable for HPLC
Sigma-Aldrich
乙腈 溶液, contains 0.1 % (v/v) formic acid, suitable for HPLC
Sigma-Aldrich
甲醇, Laboratory Reagent, ≥99.6%
Sigma-Aldrich
三氟乙酸, ≥99%, for protein sequencing
Sigma-Aldrich
乙酸, glacial, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., ≥99.8%
Supelco
甲醇, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
L -还原型谷胱甘肽, BioXtra, ≥98.0%
Supelco
谷胱甘肽, Pharmaceutical Secondary Standard; Certified Reference Material