• 主页
  • 查找结果
  • Directing valvular interstitial cell myofibroblast-like differentiation in a hybrid hydrogel platform.

Directing valvular interstitial cell myofibroblast-like differentiation in a hybrid hydrogel platform.

Advanced healthcare materials (2014-06-25)
Jesper Hjortnaes, Gulden Camci-Unal, Joshua D Hutcheson, Sung Mi Jung, Frederick J Schoen, Jolanda Kluin, Elena Aikawa, Ali Khademhosseini
摘要

Three dimensional (3D) hydrogel platforms are powerful tools, providing controllable, physiologically relevant microenvironments that could aid in understanding how various environmental factors direct valvular interstitial cell (VIC) phenotype. Continuous activation of VICs and their transformation from quiescent fibroblast to activated myofibroblast phenotype is considered to be an initiating event in the onset of valve disease. However, the relative contribution VIC phenotypes is poorly understood since most 2D culture systems lead to spontaneous VIC myofibroblastic activation. Here, a hydrogel platform composed of photocrosslinkable versions of native valvular extracellular matrix components-methacrylated hyaluronic acid (HAMA) and methacrylated gelatin (GelMA)-is proposed as a 3D culture system to study VIC phenotypic changes. These results show that VIC myofibroblast-like differentiation occurs spontaneously in mechanically soft GelMA hydrogels. Conversely, differentiation of VICs encapsulated in HAMA-GelMA hybrid hydrogels, does not occur spontaneously and requires exogenous delivery of TGFβ1, indicating that hybrid hydrogels can be used to study cytokine-dependent transition of VICs. This study demonstrates that a hybrid hydrogel platform can be used to maintain a quiescent VIC phenotype and study the effect of environmental cues on VIC activation, which will aid in understanding pathobiology of valvular disease.

材料
货号
品牌
产品描述

Sigma-Aldrich
DAPI, for nucleic acid staining
Sigma-Aldrich
氢氧化钠, reagent grade, ≥98%, pellets (anhydrous)
Sigma-Aldrich
氢氧化钠, ACS reagent, ≥97.0%, pellets
Sigma-Aldrich
氢氧化钠 溶液, 50% in H2O
Sigma-Aldrich
氢氧化钠, BioXtra, ≥98% (acidimetric), pellets (anhydrous)
Sigma-Aldrich
氢氧化钠 溶液, BioUltra, for molecular biology, 10 M in H2O
Sigma-Aldrich
氢氧化钠 溶液, 1.0 N, BioReagent, suitable for cell culture
Sigma-Aldrich
甲基丙烯酸酐, contains 2,000 ppm topanol A as inhibitor, 94%
Supelco
氢氧化钠溶液, 0.1 M NaOH in water (0.1N), Eluent concentrate for IC
Sigma-Aldrich
氢氧化钠, puriss., meets analytical specification of Ph. Eur., BP, NF, E524, 98-100.5%, pellets
Supelco
氢氧化钠 溶液, 49-51% in water, eluent for IC
Sigma-Aldrich
氢氧化钠 溶液, 5.0 M
Sigma-Aldrich
氢氧化钠, reagent grade, 97%, powder
Sigma-Aldrich
钙黄绿素-AM, BioReagent, suitable for fluorescence, ≥95.0% (HPLC)
Sigma-Aldrich
氢氧化钠, puriss. p.a., ACS reagent, reag. Ph. Eur., K ≤0.02%, ≥98%, pellets
Sigma-Aldrich
氢氧化钠, pellets, semiconductor grade, 99.99% trace metals basis
Sigma-Aldrich
钙黄绿素AM 溶液, 4 mM in DMSO, ≥90% (HPLC), solution
Sigma-Aldrich
氢氧化钠, puriss. p.a., ACS reagent, K ≤0.02%, ≥98.0% (T), pellets
Sigma-Aldrich
偶氮二甲酸二乙酯 溶液, 40 wt. % in toluene
Sigma-Aldrich
钙黄绿素-AM, Small Package (20 X 50 μg ), ≥95.0% (HPLC)
Sigma-Aldrich
氢氧化钠, beads, 20-40 mesh, reagent grade, 97%
Sigma-Aldrich
氢氧化钠, reagent grade, 97%, flakes
Sigma-Aldrich
氢氧化钠, BioUltra, for luminescence, ≥98.0% (T), pellets
Sigma-Aldrich
氢氧化钠, ultra dry, powder or crystals, 99.99% trace metals basis
Sigma-Aldrich
氢氧化钠 溶液, purum, ≥32%
Sigma-Aldrich
3-乙基-2,4-戊二酮,互变异构体的混合物, 98%
Sigma-Aldrich
氢氧化钠, anhydrous, free-flowing, Redi-Dri, reagent grade, ≥98%, pellets
Sigma-Aldrich
氢氧化钠-16O 溶液, 20 wt. % in H216O, 99.9 atom % 16O