Merck
  • Home
  • Search Results
  • Lactococcal 949 group phages recognize a carbohydrate receptor on the host cell surface.

Lactococcal 949 group phages recognize a carbohydrate receptor on the host cell surface.

Applied and environmental microbiology (2015-03-10)
Jennifer Mahony, Walter Randazzo, Horst Neve, Luca Settanni, Douwe van Sinderen
ABSTRACT

Lactococcal bacteriophages represent one of the leading causes of dairy fermentation failure and product inconsistencies. A new member of the lactococcal 949 phage group, named WRP3, was isolated from cheese whey from a Sicilian factory in 2011. The genome sequence of this phage was determined, and it constitutes the largest lactococcal phage genome currently known, at 130,008 bp. Detailed bioinformatic analysis of the genomic region encoding the presumed initiator complex and baseplate of WRP3 has aided in the functional assignment of several open reading frames (ORFs), particularly that for the receptor binding protein required for host recognition. Furthermore, we demonstrate that the 949 phages target cell wall phospho-polysaccharides as their receptors, accounting for the specificity of the interactions of these phages with their lactococcal hosts. Such information may ultimately aid in the identification of strains/strain blends that do not present the necessary saccharidic target for infection by these problematic phages.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
氯仿, contains 100-200 ppm amylenes as stabilizer, ≥99.5%
Sigma-Aldrich
氯仿, anhydrous, ≥99%, contains 0.5-1.0% ethanol as stabilizer
Sigma-Aldrich
氯仿, suitable for HPLC, ≥99.8%, contains 0.5-1.0% ethanol as stabilizer
Sigma-Aldrich
氯仿, anhydrous, contains amylenes as stabilizer, ≥99%
Sigma-Aldrich
氯仿, HPLC Plus, for HPLC, GC, and residue analysis, ≥99.9%, contains amylenes as stabilizer
Sigma-Aldrich
苯酚 溶液, Equilibrated with 10 mM Tris HCl, pH 8.0, 1 mM EDTA, BioReagent, for molecular biology
Sigma-Aldrich
苯酚, puriss. p.a., ACS reagent, reag. Ph. Eur., 99.0-100.5%
Sigma-Aldrich
苯酚, for molecular biology
Sigma-Aldrich
氯仿, contains ethanol as stabilizer, ACS reagent, ≥99.8%
Sigma-Aldrich
四环素, 98.0-102.0% (HPLC)
Sigma-Aldrich
液状苯酚, ≥89.0%
Sigma-Aldrich
苯酚, BioXtra, ≥99.5% (GC)
Sigma-Aldrich
苯酚 溶液, Saturated with 0.1 M citrate buffer, pH 4.3 ± 0.2, BioReagent, for molecular biology
Sigma-Aldrich
氯仿, puriss. p.a., reag. ISO, reag. Ph. Eur., 99.0-99.4% (GC)
Sigma-Aldrich
氯仿, suitable for HPLC, ≥99.8%, amylene stabilized
Sigma-Aldrich
L-赖氨酸 单盐酸盐, reagent grade, ≥98% (HPLC)
Sigma-Aldrich
苯酚, ≥99%
Supelco
苯酚, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
L-赖氨酸 单盐酸盐, from non-animal source, meets EP, JP, USP testing specifications, suitable for cell culture, 98.5-101.0%
Sigma-Aldrich
氯仿, contains amylenes as stabilizer, ACS reagent, ≥99.8%
Sigma-Aldrich
四环素, 98.0-102.0% (HPLC)
Sigma-Aldrich
苯酚, contains hypophosphorous as stabilizer, loose crystals, ACS reagent, ≥99.0%
Sigma-Aldrich
氯仿, HPLC Plus, for HPLC, GC, and residue analysis, ≥99.9%, contains 0.5-1.0% ethanol as stabilizer
Sigma-Aldrich
氯仿, contains ethanol as stabilizer, meets analytical specification of BP, 99-99.4% (GC)
Sigma-Aldrich
苯酚, puriss., meets analytical specification of Ph. Eur., BP, USP, 99.5-100.5% (GC)
Sigma-Aldrich
氯仿, ACS spectrophotometric grade, ≥99.8%, contains 0.5-1.0% ethanol as stabilizer
Sigma-Aldrich
苯酚, BioUltra, for molecular biology, TE-saturated, ~73% (T)
Supelco
氯仿, analytical standard
Sigma-Aldrich
苯酚, unstabilized, ReagentPlus®, ≥99%
Sigma-Aldrich
氯仿, ReagentPlus®, ≥99.8%, contains 0.5-1.0% ethanol as stabilizer