Merck
  • Home
  • Search Results
  • Compact and blinking-suppressed quantum dots for single-particle tracking in live cells.

Compact and blinking-suppressed quantum dots for single-particle tracking in live cells.

The journal of physical chemistry. B (2014-08-27)
Lucas A Lane, Andrew M Smith, Tianquan Lian, Shuming Nie
ABSTRACT

Quantum dots (QDs) offer distinct advantages over organic dyes and fluorescent proteins for biological imaging applications because of their brightness, photostability, and tunability. However, a major limitation is that single QDs emit fluorescent light in an intermittent on-and-off fashion called "blinking". Here we report the development of blinking-suppressed, relatively compact QDs that are able to maintain their favorable optical properties in aqueous solution. Specifically, we show that a linearly graded alloy shell can be grown on a small CdSe core via a precisely controlled layer-by-layer process, and that this graded shell leads to a dramatic suppression of QD blinking in both organic solvents and water. A substantial portion (>25%) of the resulting QDs does not blink (more than 99% of the time in the bright or "on" state). Theoretical modeling studies indicate that this type of linearly graded shell not only can minimize charge carrier access to surface traps but also can reduce lattice defects, both of which are believed to be responsible for carrier trapping and QD blinking. Further, we have evaluated the biological utility of blinking-suppressed QDs coated with polyethylene glycol (PEG)-based ligands and multidentate ligands. The results demonstrate that their optical properties are largely independent of surface coatings and solvating media, and that the blinking-suppressed QDs can provide continuous trajectories in live-cell receptor tracking studies.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
二甲基亚砜, Hybri-Max, sterile-filtered, BioReagent, suitable for hybridoma, ≥99.7%
Sigma-Aldrich
N,N-二甲基甲酰胺, anhydrous, 99.8%
Sigma-Aldrich
二甲基亚砜, anhydrous, ≥99.9%
Sigma-Aldrich
二甲基亚砜, for molecular biology
Sigma-Aldrich
二甲基亚砜, ACS reagent, ≥99.9%
Sigma-Aldrich
二甲基亚砜, sterile-filtered, BioPerformance Certified, meets EP, USP testing specifications, suitable for hybridoma
Sigma-Aldrich
N,N-二甲基甲酰胺, suitable for HPLC, ≥99.9%
Sigma-Aldrich
硼氢化钠, powder, ≥98.0%
Sigma-Aldrich
二甲基亚砜, suitable for HPLC, ≥99.7%
Sigma-Aldrich
二甲基亚砜, ReagentPlus®, ≥99.5%
Sigma-Aldrich
N,N-二甲基甲酰胺, ACS reagent, ≥99.8%
Sigma-Aldrich
庚烷, suitable for HPLC, ≥99%
Sigma-Aldrich
二甲基亚砜, ≥99.5% (GC), suitable for plant cell culture
Sigma-Aldrich
油胺, technical grade, 70%
Sigma-Aldrich
N-羟基丁二酰亚胺, 98%
Sigma-Aldrich
硼氢化钠, ReagentPlus®, 99%
Sigma-Aldrich
4-(二甲氨基)吡啶, ReagentPlus®, ≥99%
Sigma-Aldrich
庚烷, anhydrous, 99%
Sigma-Aldrich
庚烷, ReagentPlus®, 99%
Sigma-Aldrich
二甲基亚砜, meets EP testing specifications, meets USP testing specifications
Sigma-Aldrich
二甲基亚砜, puriss. p.a., ACS reagent, ≥99.9% (GC)
Sigma-Aldrich
N,N-二甲基甲酰胺, for molecular biology, ≥99%
Sigma-Aldrich
1-硫代甘油, liquid, BioReagent, suitable for cell culture, ≥97% (titration)
Sigma-Aldrich
硒, powder, −100 mesh, 99.99% trace metals basis
Sigma-Aldrich
三正辛基膦, 97%
Sigma-Aldrich
N,N-二甲基甲酰胺, ReagentPlus®, ≥99%
Sigma-Aldrich
二甲基亚砜, BioUltra, for molecular biology, ≥99.5% (GC)
Sigma-Aldrich
1-硫代甘油, ≥97%
Sigma-Aldrich
硼氢化钠, granular, 99.99% trace metals basis
Sigma-Aldrich
硒, powder, −100 mesh, ≥99.5% trace metals basis