• 主页
  • 查找结果
  • Induction of Th17 cells by segmented filamentous bacteria in the murine intestine.

Induction of Th17 cells by segmented filamentous bacteria in the murine intestine.

Journal of immunological methods (2015-04-11)
Adam M Farkas, Casandra Panea, Yoshiyuki Goto, Gaku Nakato, Marta Galan-Diez, Seiko Narushima, Kenya Honda, Ivaylo I Ivanov
摘要

Segmented filamentous bacteria (SFB) are Gram-positive, anaerobic, spore-forming commensals that reside in the gut of many animal species. Described more than forty years ago, SFB have recently gained interest due to their unique ability to modulate the host immune system through induction of IgA and Th17 cells. Here, we describe a collection of methods to detect and quantify SFB and SFB adhesion in intestinal mucosa, as well as SFB-specific CD4 T cells in the lamina propria. In addition, we describe methods for purification of SFB from fecal material of SFB-monoassociated gnotobiotic mice. Using these methods we examine the kinetics of SFB colonization and Th17 cell induction. We also show that SFB colonize unevenly the intestinal mucosa and that SFB adherence occurs predominantly in the terminal ileum and correlates with an increased proportion of SFB-specific Th17 cells.

材料
货号
品牌
产品描述

Sigma-Aldrich
氯化钠, BioXtra, ≥99.5% (AT)
Sigma-Aldrich
氯化钠, for molecular biology, DNase, RNase, and protease, none detected, ≥99% (titration)
Sigma-Aldrich
氯化钠, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99%
Sigma-Aldrich
氯化钠 溶液, 0.9% in water, BioXtra, suitable for cell culture
Sigma-Aldrich
氯化钠 溶液, 5 M in H2O, BioReagent, for molecular biology, suitable for cell culture
Sigma-Aldrich
氯化钠 溶液, 5 M
SAFC
氯化钠 溶液, 5 M
Sigma-Aldrich
氯化钠, meets analytical specification of Ph. Eur., BP, USP, 99.0-100.5%
Sigma-Aldrich
氧化锆 (IV), powder, 5 μm, 99% trace metals basis
Sigma-Aldrich
氯化钠, BioUltra, for molecular biology, ≥99.5% (AT)
Sigma-Aldrich
氯化钠 溶液, 0.85%
Sigma-Aldrich
氯化钠 溶液, BioUltra, for molecular biology, ~5 M in H2O
Sigma-Aldrich
5(6)-羧基二乙酸荧光素 N-琥珀酰亚胺酯, BioReagent, suitable for fluorescence, ≥90% (HPLC)
Sigma-Aldrich
氯化钠, 99.999% trace metals basis
Sigma-Aldrich
氧化锆 (IV), nanopowder, <100 nm particle size (TEM)
Sigma-Aldrich
氯化钠, BioPerformance Certified, ≥99% (titration), suitable for insect cell culture, suitable for plant cell culture
Sigma-Aldrich
氯化钠, tablet
Sigma-Aldrich
氧化锆(IV), nanoparticles, dispersion, <100 nm particle size (BET), 5 wt. % in H2O
Sigma-Aldrich
氯化钠, AnhydroBeads, −10 mesh, 99.999% trace metals basis
Sigma-Aldrich
氧化锆 (IV), 99.99% trace metals basis (purity excludes ~2% HfO2)
Sigma-Aldrich
氯化钠, random crystals, optical grade, 99.9% trace metals basis
Sigma-Aldrich
氯化钠, AnhydroBeads, −10 mesh, 99.99% trace metals basis
Sigma-Aldrich
5-Carboxy-fluorescein diacetate N-succinimidyl ester, for fluorescence, ≥95.0% (HPLC)
Sigma-Aldrich
氧化锆(IV), nanoparticles, dispersion, <100 nm particle size (BET), 10 wt. % in H2O
Sigma-Aldrich
氯化钠-35Cl, 99 atom % 35Cl