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Production, extraction and stabilization of lutein from microalga Chlorella sorokiniana MB-1.

Bioresource technology (2015-11-01)
Chun-Yen Chen, Jesisca, Chienyan Hsieh, Duu-Jong Lee, Chien-Hsiang Chang, Jo-Shu Chang
ABSTRACT

The efficiencies of extraction and preservation of lutein from microalgae are critical for the success of its commercialization. In this study, lutein was produced by Chlorella sorokiniana MB-1 via semi-batch mixotrophic cultivation. The microalgal biomass with a lutein content of 5.21mg/g was pretreated by bead-beating and high pressure cell disruption methods, and the lutein content was harvested by a reduced pressure extraction method. The effect of pretreatment, pressure, solvent type, extraction time and temperature on lutein recovery was investigated. Using high pressure pretreatment followed by extraction with tetrahydrofuran (THF) as solvent resulted in high lutein recovery efficiencies of 87.0% (20min) and 99.5% (40min) at 850mbar and 25°C. In contrast, using ethanol as the solvent, 86.2% lutein recovery was achieved under 450mbar, 35°C and 40min extraction. The extracted lutein was stabilized in olive oil or sunflower oil with half-lives of 53.1 and 63.8days, respectively.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
四氢呋喃, anhydrous, ≥99.9%, inhibitor-free
Sigma-Aldrich
四氢呋喃, anhydrous, contains 250 ppm BHT as inhibitor, ≥99.9%
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二乙醚, contains 1 ppm BHT as inhibitor, anhydrous, ≥99.7%
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二乙醚, anhydrous, ACS reagent, ≥99.0%, contains BHT as inhibitor
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四氢呋喃, contains 250 ppm BHT as inhibitor, ACS reagent, ≥99.0%
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二乙醚, ACS reagent, anhydrous, ≥99.0%, contains BHT as inhibitor
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四氢呋喃, contains 250 ppm BHT as inhibitor, puriss. p.a., ACS reagent, reag. Ph. Eur., ≥99.9%
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二乙醚, contains BHT as inhibitor, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., ≥99.8%
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四氢呋喃, suitable for HPLC, ≥99.9%, inhibitor-free
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四氢呋喃, ReagentPlus®, ≥99.0%, contains 250 ppm BHT as inhibitor
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二乙醚, ACS reagent, ≥98.0%, contains ≤2% ethanol and ≤10ppm BHT as inhibitor
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叶黄素, from marigold
Sigma-Aldrich
四氢呋喃, ACS reagent, ≥99.0%, contains 250 ppm BHT as inhibitor
Sigma-Aldrich
四氢呋喃, contains 250 ppm BHT as inhibitor, ACS reagent, ≥99.0%
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二乙醚, reagent grade, ≥98%, contains ≤2% ethanol and ≤10ppm BHT as inhibitor
Sigma-Aldrich
二乙醚, puriss., contains ~5 mg/L 2,6-di-tert.-butyl-4-methylphenol as stabilizer, meets analytical specification of Ph. Eur., BP, ≥99.5% (GC)