Merck
  • Home
  • Search Results
  • Self-assembly micelles with lipid core of cholesterol for docetaxel delivery to B16F10 melanoma and HepG2 cells.

Self-assembly micelles with lipid core of cholesterol for docetaxel delivery to B16F10 melanoma and HepG2 cells.

Journal of liposome research (2014-10-03)
Jaleh Varshosaz, Somayeh Taymouri, Farshid Hassanzadeh, Shaghaiegh Haghjooy Javanmard, Mahboobeh Rostami
ABSTRACT

The objective of the present study was to prepare a micellar polymeric carrier for the delivery of Docetaxel (DTX) as a kind of polysorbate free preparation. Pluronic F127 (PF127) was conjugated to cholesterol (Chol) via succinyl linkage and characterized by FTIR and HNMR. DTX-loaded polymeric micelles were prepared via film hydration method. Physicochemical properties of micelles including particle size, zeta potential, drug loading and release efficiency were studied. The critical micelle concentration (CMC) was determined using pyrene as a hydrophobic fluorescent probe. In vitro cytotoxicity of micelles was evaluated in B16F10 melanoma cells and HepG2 cell line. The FTIR and HNMR spectroscopy methods confirmed the conjugation of PF127 to cholesterol via succinyl linkage. The micelles were spherical under scanning electron microscope (SEM) with the mean particle size of 248.2 ± 8-278.8 ± 12.3 nm and zeta potential ranging from -17.2 ± 8.7 to -28.4 ± 12.7 mV. Drug loading efficiency was higher than 98%. The in vitro release study showed the sustained release behavior of DTX within 144 h. The CMC of the micelles was about 41.67 ± 0.17 µg/ml, which was significantly lower than the CMC of pure PF127 micelles. Compared with the free drug, DTX-loaded micelles showed higher cytotoxicity against B16F10 melanoma and HepG2 cell lines.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
二甲基亚砜, Hybri-Max, sterile-filtered, BioReagent, suitable for hybridoma, ≥99.7%
Sigma-Aldrich
二甲基亚砜, anhydrous, ≥99.9%
Sigma-Aldrich
二甲基亚砜, for molecular biology
Sigma-Aldrich
二氯甲烷, anhydrous, ≥99.8%, contains 40-150 ppm amylene as stabilizer
Sigma-Aldrich
乙腈, anhydrous, 99.8%
Sigma-Aldrich
甲醇, anhydrous, 99.8%
Sigma-Aldrich
二甲基亚砜, sterile-filtered, BioPerformance Certified, meets EP, USP testing specifications, suitable for hybridoma
Sigma-Aldrich
二甲基亚砜, ≥99.5% (GC), suitable for plant cell culture
Sigma-Aldrich
胆固醇, Sigma Grade, ≥99%
Sigma-Aldrich
噻唑蓝, powder, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥97.5% (HPLC)
Sigma-Aldrich
噻唑蓝, 98%
Sigma-Aldrich
4-(二甲氨基)吡啶, ReagentPlus®, ≥99%
Sigma-Aldrich
N,N′-二环己基碳二亚胺, 99%
Sigma-Aldrich
二甲基亚砜, meets EP testing specifications, meets USP testing specifications
Sigma-Aldrich
胆固醇, powder, BioReagent, suitable for cell culture, ≥99%
Sigma-Aldrich
丁二酸酐, ≥99% (GC)
Sigma-Aldrich
乙腈 溶液, contains 0.1 % (v/v) trifluoroacetic acid, suitable for HPLC
SAFC
胆固醇、植物的, SyntheChol®, NF, Ph. Eur., JP
Sigma-Aldrich
二甲基亚砜, BioUltra, for molecular biology, ≥99.5% (GC)
Sigma-Aldrich
乙腈 溶液, contains 0.1 % (v/v) formic acid, suitable for HPLC
Sigma-Aldrich
芘, 98%
Sigma-Aldrich
SyntheChol ® NS0 补充, 500 ×, synthetic cholesterol, animal component-free, aqueous solution, sterile-filtered, suitable for cell culture
Sigma-Aldrich
二氯甲烷, suitable for HPLC, ≥99.9%, contains 40-150 ppm amylene as stabilizer
Sigma-Aldrich
二甲基亚砜, PCR Reagent
Sigma-Aldrich
胆固醇, from sheep wool, ≥92.5% (GC), powder
Sigma-Aldrich
乙腈, HPLC Plus, ≥99.9%, poly-coated bottles
Sigma-Aldrich
甲醇, HPLC Plus, ≥99.9%, poly-coated bottles
Sigma-Aldrich
芘, puriss. p.a., for fluorescence, ≥99.0% (GC)
Sigma-Aldrich
N,N′-二环己基碳二亚胺 溶液, 1.0 M in methylene chloride
Sigma-Aldrich
乙腈, electronic grade, 99.999% trace metals basis