Merck
  • Home
  • Search Results
  • Polarized endosome dynamics by spindle asymmetry during asymmetric cell division.

Polarized endosome dynamics by spindle asymmetry during asymmetric cell division.

Nature (2015-12-15)
Emmanuel Derivery, Carole Seum, Alicia Daeden, Sylvain Loubéry, Laurent Holtzer, Frank Jülicher, Marcos Gonzalez-Gaitan
ABSTRACT

During asymmetric division, fate determinants at the cell cortex segregate unequally into the two daughter cells. It has recently been shown that Sara (Smad anchor for receptor activation) signalling endosomes in the cytoplasm also segregate asymmetrically during asymmetric division. Biased dispatch of Sara endosomes mediates asymmetric Notch/Delta signalling during the asymmetric division of sensory organ precursors in Drosophila. In flies, this has been generalized to stem cells in the gut and the central nervous system, and, in zebrafish, to neural precursors of the spinal cord. However, the mechanism of asymmetric endosome segregation is not understood. Here we show that the plus-end kinesin motor Klp98A targets Sara endosomes to the central spindle, where they move bidirectionally on an antiparallel array of microtubules. The microtubule depolymerizing kinesin Klp10A and its antagonist Patronin generate central spindle asymmetry. This asymmetric spindle, in turn, polarizes endosome motility, ultimately causing asymmetric endosome dispatch into one daughter cell. We demonstrate this mechanism by inverting the polarity of the central spindle by polar targeting of Patronin using nanobodies (single-domain antibodies). This spindle inversion targets the endosomes to the wrong cell. Our data uncover the molecular and physical mechanism by which organelles localized away from the cellular cortex can be dispatched asymmetrically during asymmetric division.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
甘油, for molecular biology, ≥99.0%
Sigma-Aldrich
羟乙基哌嗪乙硫磺酸, ≥99.5% (titration)
Sigma-Aldrich
氯化钠, BioXtra, ≥99.5% (AT)
Sigma-Aldrich
二乙醚, contains 1 ppm BHT as inhibitor, anhydrous, ≥99.7%
Sigma-Aldrich
羟乙基哌嗪乙硫磺酸, BioPerformance Certified, ≥99.5% (titration), suitable for cell culture
Sigma-Aldrich
甘油, ≥99.5%
Sigma-Aldrich
IPTG, ≥99% (TLC), ≤0.1% Dioxane
Sigma-Aldrich
二乙醚, anhydrous, ACS reagent, ≥99.0%, contains BHT as inhibitor
Sigma-Aldrich
甘油, ACS reagent, ≥99.5%
Sigma-Aldrich
甘油, ReagentPlus®, ≥99.0% (GC)
Sigma-Aldrich
氯化镁 溶液, for molecular biology, 1.00 M±0.01 M
Sigma-Aldrich
氯化钠, for molecular biology, DNase, RNase, and protease, none detected, ≥99% (titration)
Sigma-Aldrich
氯化镁, anhydrous, ≥98%
Sigma-Aldrich
DL-二硫代苏糖醇 溶液, BioUltra, for molecular biology, ~1 M in H2O
Sigma-Aldrich
氯化钠 溶液, 5 M in H2O, BioReagent, for molecular biology, suitable for cell culture
Sigma-Aldrich
氯化钙 溶液, BioUltra, for molecular biology, ~1 M in H2O
Sigma-Aldrich
氯化钠 溶液, 0.9% in water, BioXtra, suitable for cell culture
Sigma-Aldrich
苯甲磺酰氟, ≥98.5% (GC)
SAFC
氯化钠 溶液, 5 M
Sigma-Aldrich
氯化钠, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99%
Sigma-Aldrich
乙二醇-双(2-氨基乙醚)-N,N,N′,N′-四乙酸, for molecular biology, ≥97.0%
Sigma-Aldrich
氯化钠 溶液, 5 M
Sigma-Aldrich
氟化钠, ACS reagent, ≥99%
Sigma-Aldrich
二乙醚, ACS reagent, anhydrous, ≥99.0%, contains BHT as inhibitor
Supelco
DL-二硫代苏糖醇 溶液, 1 M in H2O
Sigma-Aldrich
2-二(2-羟乙基)氨基-2-羟甲基-1,3-丙二醇, ≥98.0% (titration)
Sigma-Aldrich
甘油, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for electrophoresis, ≥99% (GC)
Sigma-Aldrich
哌嗪-N,N'-二(2-乙磺酸), ≥99% (titration)
Sigma-Aldrich
HEPES缓冲溶液, 1 M in H2O
Sigma-Aldrich
甘油, meets USP testing specifications