• 主页
  • 查找结果
  • Propofol-induced pain sensation involves multiple mechanisms in sensory neurons.

Propofol-induced pain sensation involves multiple mechanisms in sensory neurons.

Pflugers Archiv : European journal of physiology (2014-10-11)
Rei Nishimoto, Makiko Kashio, Makoto Tominaga
摘要

Propofol, a commonly used intravenous anesthetic agent, is known to at times cause pain sensation upon injection in humans. However, the molecular mechanisms underlying this effect are not fully understood. Although propofol was reported to activate human transient receptor potential ankyrin 1 (TRPA1) in this regard, its action on human TRP vanilloid 1 (TRPV1), another nociceptive receptor, is unknown. Furthermore, whether propofol activates TRPV1 in rodents is controversial. Here, we show that propofol activates human and mouse TRPA1. In contrast, we did not observe propofol-evoked human TRPV1 activation, while the ability of propofol to activate mouse TRPV1 was very small. We also found that propofol caused increases in intracellular Ca(2+) concentrations in a considerable portion of dorsal root ganglion (DRG) cells from mice lacking both TRPV1 and TRPA1, indicating the existence of TRPV1- and TRPA1-independent mechanisms for propofol action. In addition, propofol produced action potential generation in a type A γ-amino butyric acid (GABAA) receptor-dependent manner. Finally, we found that both T-type and L-type Ca(2+) channels are activated downstream of GABAA receptor activation by propofol. Thus, we conclude that propofol may cause pain sensation through multiple mechanisms involving not only TRPV1 and TRPA1 but also voltage-gated channels downstream of GABAA receptor activation.

材料
货号
品牌
产品描述

Sigma-Aldrich
氢氧化钠 溶液, BioUltra, for molecular biology, 10 M in H2O
Sigma-Aldrich
氢氧化钠 溶液, 1.0 N, BioReagent, suitable for cell culture
Sigma-Aldrich
辣椒素, ≥95%, from Capsicum sp.
Sigma-Aldrich
丁酸, ≥99%
Sigma-Aldrich
(±)-维拉帕米 盐酸盐, ≥99% (titration), powder
Sigma-Aldrich
丁酸, natural, ≥99%, FCC, FG
Sigma-Aldrich
硝苯地平, ≥98% (HPLC), powder
Sigma-Aldrich
辣椒素, from Capsicum sp., ≥50% (HPLC)
Sigma-Aldrich
辣椒素, natural
Sigma-Aldrich
(+)-荷包牡丹碱, ≥97.0% (TLC)
Sigma-Aldrich
丁酸, ≥99%, FG
Sigma-Aldrich
2,6-二异丙基苯酚, 97%
Sigma-Aldrich
氢氧化钠, BioUltra, for luminescence, ≥98.0% (T), pellets
Sigma-Aldrich
氢氧化钠, ultra dry, powder or crystals, 99.99% trace metals basis
Sigma-Aldrich
氟桂嗪 二盐酸盐, ≥98% (TLC)
Sigma-Aldrich
3-乙基-2,4-戊二酮,互变异构体的混合物, 98%
Sigma-Aldrich
NNC 55-0396 hydrate, ≥98% (HPLC)
Sigma-Aldrich
氢氧化钠-16O 溶液, 20 wt. % in H216O, 99.9 atom % 16O