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  • Validation approach for a fast and simple targeted screening method for 75 antibiotics in meat and aquaculture products using LC-MS/MS.

Validation approach for a fast and simple targeted screening method for 75 antibiotics in meat and aquaculture products using LC-MS/MS.

Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment (2016-09-03)
Estelle Dubreil, Sophie Gautier, Marie-Pierre Fourmond, Mélaine Bessiral, Murielle Gaugain, Eric Verdon, Dominique Pessel
摘要

An approach is described to validate a fast and simple targeted screening method for antibiotic analysis in meat and aquaculture products by LC-MS/MS. The strategy of validation was applied for a panel of 75 antibiotics belonging to different families, i.e., penicillins, cephalosporins, sulfonamides, macrolides, quinolones and phenicols. The samples were extracted once with acetonitrile, concentrated by evaporation and injected into the LC-MS/MS system. The approach chosen for the validation was based on the Community Reference Laboratory (CRL) guidelines for the validation of screening qualitative methods. The aim of the validation was to prove sufficient sensitivity of the method to detect all the targeted antibiotics at the level of interest, generally the maximum residue limit (MRL). A robustness study was also performed to test the influence of different factors. The validation showed that the method is valid to detect and identify 73 antibiotics of the 75 antibiotics studied in meat and aquaculture products at the validation levels.

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Sigma-Aldrich
五氟丙酸, 97%
Sigma-Aldrich
2,2,3,3,3-五氟丙基丙烯酸酯, 98%, contains 100 ppm 4-tert-butylcatechol as inhibitor
Sigma-Aldrich
2,2,3,3,4,4,4-七氟丁基丙烯酸酯, 97%