Merck
  • Home
  • Search Results
  • Stromagenesis during tumorigenesis: characterization of tumor-associated fibroblasts and stroma-derived 3D matrices.

Stromagenesis during tumorigenesis: characterization of tumor-associated fibroblasts and stroma-derived 3D matrices.

Methods in molecular biology (Clifton, N.J.) (2009-02-28)
Remedios Castelló-Cros, Edna Cukierman
ABSTRACT

It is increasingly recognized that interactions between cancer cells and their surrounding stroma are critical for promoting the growth and invasiveness of tumors. For example, cancer cells alter the topography and molecular composition of stromal extracellular matrix by increasing paracrine regulation of fibroblastic stromal cells during early tumor development. In turn, these physical and biochemical alterations of the stroma, profoundly affect the properties of the cancer cells. However, little is known about the cross-talk between stroma and cancer cells, and it is mainly due to the lack of a suitable in vitro system to mimic the stroma in vivo. We present an in vivo-like 3D stromal system derived from fibroblasts harvested from tissue samples representing various stages of stroma progression during tumorigenesis. The chapter describes how to isolate and characterize fibroblasts from a plethora of tissue samples. It describes how to produce and characterize fibroblast-derived 3D matrices. Finally, it describes how to test matrix permissiveness by analyzing the morphology of cancer cells cultured within various 3D matrices.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
二甲基亚砜, Hybri-Max, sterile-filtered, BioReagent, suitable for hybridoma, ≥99.7%
Sigma-Aldrich
甲醇, suitable for HPLC, ≥99.9%
Sigma-Aldrich
二甲基亚砜, for molecular biology
Sigma-Aldrich
二甲基亚砜, anhydrous, ≥99.9%
Sigma-Aldrich
甲醇, anhydrous, 99.8%
Sigma-Aldrich
TWEEN ® 20, viscous liquid
Sigma-Aldrich
二甲基亚砜, ACS reagent, ≥99.9%
Sigma-Aldrich
二甲基亚砜, sterile-filtered, BioPerformance Certified, meets EP, USP testing specifications, suitable for hybridoma
Sigma-Aldrich
Triton X-100, laboratory grade
Sigma-Aldrich
甲醇, ACS reagent, ≥99.8%
Sigma-Aldrich
蔗糖, for molecular biology, ≥99.5% (GC)
Sigma-Aldrich
Triton X-100, for molecular biology
Sigma-Aldrich
TWEEN ® 20, for molecular biology, viscous liquid
Sigma-Aldrich
2-巯基乙醇, for molecular biology, suitable for electrophoresis, suitable for cell culture, BioReagent, 99% (GC/titration)
Sigma-Aldrich
二甲基亚砜, ReagentPlus®, ≥99.5%
Sigma-Aldrich
2-巯基乙醇, ≥99.0%
Sigma-Aldrich
TWEEN ® 20, BioXtra, viscous liquid
Sigma-Aldrich
二甲基亚砜, suitable for HPLC, ≥99.7%
Sigma-Aldrich
二甲基亚砜, ≥99.5% (GC), suitable for plant cell culture
Sigma-Aldrich
蔗糖, ≥99.5% (GC)
Sigma-Aldrich
Triton X-100, BioXtra
Sigma-Aldrich
甲醇, HPLC Plus, ≥99.9%
Sigma-Aldrich
L -抗坏血酸, 99%
Sigma-Aldrich
丽春红S染液(零售包装) 溶液, BioReagent, suitable for electrophoresis, 0.1 % (w/v) in 5% acetic acid
Sigma-Aldrich
甲醇, suitable for HPLC, gradient grade, ≥99.9%
Sigma-Aldrich
戊二醛 溶液, Grade II, 25% in H2O
Sigma-Aldrich
蔗糖, BioXtra, ≥99.5% (GC)
Sigma-Aldrich
L -抗坏血酸, powder, suitable for cell culture, γ-irradiated
Sigma-Aldrich
戊二醛 溶液, Grade I, 25% in H2O, specially purified for use as an electron microscopy fixative
Sigma-Aldrich
蔗糖, BioUltra, for molecular biology, ≥99.5% (HPLC)