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  • Impact of aeration and heme-activated respiration on Lactococcus lactis gene expression: identification of a heme-responsive operon.

Impact of aeration and heme-activated respiration on Lactococcus lactis gene expression: identification of a heme-responsive operon.

Journal of bacteriology (2008-05-20)
Martin Bastian Pedersen, Christel Garrigues, Karine Tuphile, Célia Brun, Karin Vido, Mads Bennedsen, Henrik Møllgaard, Philippe Gaudu, Alexandra Gruss
摘要

Lactococcus lactis is a widely used food bacterium mainly characterized for its fermentation metabolism. However, this species undergoes a metabolic shift to respiration when heme is added to an aerobic medium. Respiration results in markedly improved biomass and survival compared to fermentation. Whole-genome microarrays were used to assess changes in L. lactis expression under aerobic and respiratory conditions compared to static growth, i.e., nonaerated. We observed the following. (i) Stress response genes were affected mainly by aerobic fermentation. This result underscores the differences between aerobic fermentation and respiration environments and confirms that respiration growth alleviates oxidative stress. (ii) Functions essential for respiratory metabolism, e.g., genes encoding cytochrome bd oxidase, menaquinone biosynthesis, and heme uptake, are similarly expressed under the three conditions. This indicates that cells are prepared for respiration once O(2) and heme become available. (iii) Expression of only 11 genes distinguishes respiration from both aerobic and static fermentation cultures. Among them, the genes comprising the putative ygfCBA operon are strongly induced by heme regardless of respiration, thus identifying the first heme-responsive operon in lactococci. We give experimental evidence that the ygfCBA genes are involved in heme homeostasis.

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Sigma-Aldrich
二甲基亚砜, for molecular biology
Sigma-Aldrich
变溶菌素 来源于球孢链霉菌 ATCC 21553, lyophilized powder, ≥4000 units/mg protein (biuret), Chromatographically purified
Sigma-Aldrich
变溶菌素 来源于球孢链霉菌 ATCC 21553, free of DNA contaminants, suitable for Microbiome research, lyophilized powder, ≥4000 units/mg protein (biuret)