High-Performance Silicon Nanopore Hemofiltration Membranes.

Journal of membrane science (2010-01-08)
William H Fissell, Anna Dubnisheva, Abigail N Eldridge, Aaron J Fleischman, Andrew L Zydney, Shuvo Roy
摘要

Silicon micromachining provides the precise control of nanoscale features that can be fundamentally enabling for miniaturized, implantable medical devices. Concerns have been raised regarding blood biocompatibility of silicon-based materials and their application to hemodialysis and hemofiltration. A high-performance ultrathin hemofiltration membrane with monodisperse slit-shaped pores was fabricated using a sacrificial oxide technique and then surface-modified with poly(ethylene glycol) (PEG). Fluid and macromolecular transport matched model predictions well. Protein adsorption, fouling, and thrombosis were significantly inhibited by the PEG. The membrane retained hydraulic permeability and molecular selectivity during a 90 hour hemofiltration experiment with anticoagulated bovine whole blood. This is the first report of successful prolonged hemofiltration with a silicon nanopore membrane. The results demonstrate feasibility of renal replacement devices based on these membranes and materials.

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Sigma-Aldrich
碳酸酐酶 来源于牛红细胞, lyophilized powder, ≥2,000 W-A units/mg protein
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碳酸酐酶 来源于牛红细胞, ≥95% (SDS-PAGE), specific activity ≥3,500 W-A units/mg protein, lyophilized powder
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Thyroglobulin 来源于牛甲状腺, powder, ≥90% (agarose gel electrophoresis)
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白蛋白 来源于鸡蛋白, lyophilized powder, ≥90% (agarose gel electrophoresis)
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碳酸酐酶 来源于牛红细胞, BioReagent, suitable for GFC marker
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碳酸酐酶 来源于牛红细胞, For use as a marker in SDS-PAGE
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碳酸酐酶 来源于牛红细胞, non-denaturing PAGE marker