Merck
  • 主页
  • 查找结果
  • Enhanced surface acoustic wave cell sorting by 3D microfluidic-chip design.

Enhanced surface acoustic wave cell sorting by 3D microfluidic-chip design.

Lab on a chip (2017-10-11)
W L Ung, K Mutafopulos, P Spink, R W Rambach, T Franke, D A Weitz
摘要

We demonstrate an acoustic wave driven microfluidic cell sorter that combines advantages of multilayer device fabrication with planar surface acoustic wave excitation. We harness the strong vertical component of the refracted acoustic wave to enhance cell actuation by using an asymmetric flow field to increase cell deflection. Precise control of the 3-dimensional flow is realized by topographical structures implemented on the top of the microchannel. We experimentally quantify the effect of the structure dimensions and acoustic parameter. The design attains cell sorting rates and purities approaching those of state of the art fluorescence-activated cell sorters with all the advantages of microfluidic cell sorting.

材料
货号
品牌
产品描述

Sigma-Aldrich
磷酸盐缓冲盐水, powder, pH 7.4, for preparing 1 L solutions
Sigma-Aldrich
OptiPrep密度梯度培养基, used for cell and subcellular organelle isolation
Sigma-Aldrich
钙黄绿素, Used for the fluorometric determination of calcium and EDTA titration of calcium in the presence of magnesium.
Sigma-Aldrich
同二聚乙胺, suitable for fluorescence, ~90% (HPCE)