1 base (NaOt-Bu, 2 M in THF)
2 solvents (1,4 Dioxane and DMAc)
12 pre-weighed catalysts
Materials required - set up:
Additional (not included) items needed:
Solutions & slurries to make: Go to the online user set-up page to enter molecular weights into the downloadable excel file.
Set Up Procedure:
(See KitAlysis™ video for useful visuals)
Quench Solution Recipe
-49 mL CH3CN
-1 mL AcOH
-15.4 mg Biphenyl (KitAlysis™ Internal Standard provided in the kit)
Note: This recipe makes 50 mL which is enough stock solution for all four screening set in the KitAlysis™ Buchwald-Hartwig Amination Reaction Screening Kit
Work-up Procedure and Analysis
Sealing the Plate-screwing down the cover
Sealing the plate properly is critical to success. The key is light even pressure to the lid of the block to keep the cover flat while sealing.
Probe for hot plate does not fit into reaction block hole
Use a small oil bath or other metal block (for best results) in the back of the hot plate and place the probe in there. Place reaction block as close to the center of the hot plate as possible for more even stirring.
Due to narrow opening of pipette tips, slurry additions will result in blockages. To aid in uniform dosing, snip off the end of the tip at the first marker (~10 mm). To ensure an evenly dispersed aliquot, it is critical that the mixture is stirring while you draw aliquots for dosing into the corresponding reaction vial.