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Scalable Tangential Flow Filtration (TFF) for Downstream Processing of Viral Vectors for Gene Therapy and Vaccine Production

Our overview of scalable tangential flow filtration for viral vector production will provide key considerations for developing and optimizing your downstream process.

Renewable Electricity Toolkit

The Renewable Electricity Supplier Toolkit helps suppliers learn renewable electricity basics and how to bring projects to life.

Transforming the Biopharma Supply Chain and Analytics Capabilities with eData

An essential step in the progress towards Pharma 4.0 is the conversion of paper-based data to an electronic format, allowing it to be transferred, received, and available at the right time, in the right place, by those who need it.

Membrane Prefilters Improve Sterilizing Filter Capacity and Process Economics in Bioprocessing

Prefilters can be used throughout bioprocessing to remove to remove particulates, improve quality and protect sterilizing filters and downstream unit operations.

Viral Safety in Bioprocessing

Viral safety in bioprocessing relies on a framework of (1) preventing contamination, (2) detecting contamination, and (3) removing or inactivating viral contaminants.

Bioburden Control in Bioprocessing

Bioburden control in bioprocessing requires assessing the risks, mitigating the risks, and monitoring bioburden levels.

Prefilters and Sterilizing-grade Filter Formats: Selection Guide

Aseptic filters are offered in disposable capsules and cartridge formats. This guide will help you find the right format for your bioburden control.

Sterilizing Grade Filtration Unit Operations for Plasmid DNA Processes

We discuss the final filtration step of plasmid DNA manufacturing and its key considerations and challenges. Download our free pDNA downstream purification eBook or ask our experts.

Viscosity Reduction in High Concentration TFF

This overview describes how use of viscosity-reducing excipients can address viscosity challenges in high concentration tangential flow filtration (TFF) and improve process economics.

Tangential Flow Filtration in the Antibody Drug Conjugate (ADC) Manufacturing Process

Find best practices for using Pellicon® Capsules in ADC processing as well as data from two studies using Pellicon® Capsules and Pellicon® cassettes in the ADC manufacturing process.

Custom Oligos Modifications

We offer over 200 custom DNA oligos modification for both DNA, RNA oligonucleotides and sequencing to probes for gene.

Oligonucleotide Quality Control by Mass Spectrometry

Mass Spectrometry is the technology of choice for analyzing oligonucleotide synthesis. It enables the most sensitive detection of low levels of by-products, which can affect performance.

PCR/qPCR Data Analysis

After a traditional PCR has been completed, the PCR/qPCR data analysis is conducted by resolution through an agarose gel or, more recently, through a capillary.

Dual-Labeled Probes

Dual-Labeled Probes are the most common probe type for qPCR and are often referred to as hydrolysis probes.

How qPCR Works

Watch these videos to learn how real time or quantitative PCR (qPCR) works and the benefits of both the SYBR Green-based and probe-based methods of qPCR assay.

Direct and Crude Extract qPCR

Rapid qPCR analysis from highly inhibited tissue and blood sample extractions with KAPA PROBE.

Kapa Biosystems qPCR Reagents and Kits

Quantitative PCR (qPCR) and qRT-PCR reagents and kits that are designed to deliver maximum efficiency and reliable results from a variety of genetic materials.

KAPA SYBR® FAST qPCR Kits FAQs

Review some of the most asked questions regarding the KAPA SYBR FAST qPCR kits, which provide optimal performance under strict real-time PCR reaction conditions, and improve the signal-to-noise ratio (fluorescence), quantitation cycle (Cq), linearity and sensitivity.

KiCqStart^® SYBR^® Green Primers FAQs

Primer pairs detect prevalent eukaryotic genes, but may co-amplify other transcripts, emphasizing the importance of comprehensive primer design.

KiCqStart^® Probe Assays Frequently Asked Questions

MIQE: Real-Time PCR Experiment Standards

MIQE-compliant qPCR services support primer design, protocol development, and data analysis for faster product development and publication.

Molecular Beacons

Learn about precision Sigma-Aldrich Molecular Beacon oligonucleotide probes used for sequence detection in qPCR for in vitro research.

Primer and Probe Design

Video: Primer and Probe Design

Probe-based qPCR

Probe-based QPCR offers high sensitivity and specificity with fluorescent-labeled probes and various dye options for multiplex amplification.

Probe QPCR for Gene-Level Expression

qPCR investigates gene expression, amplification, and alterations, crucial for tumor biology and understanding cancer genetics.

Quantitative PCR Basics

Real-time polymerase chain reaction allows researchers to estimate the quantity of starting material in a sample. It has a much wider dynamic range of analysis than conventional PCR

Quantitative PCR and Digital PCR Detection Methods

Fluorescent dyes or probes are included in PCR mixes to monitor the change in NA amplicon concentration as the reaction proceeds.

Quantitative RT-PCR

RT-qPCR products combine the effective of Reverse Transcriptase with hot-start taq-directed antibody in convenient ReadyMixes for probe-based or SYBR® Green based applications.

RNA Immunoprecipitation (RIP) FAQs

RNA Immunoprecipitation (RIP) Frequently Asked Questions

Universal ProbeLibrary System Technology

UPL is based on only 165 short hydrolysis probes. They are labeled at the 5' end with fluorescein (FAM) and at the 3' end with a dark quencher dye.

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