We offer a complete line of products and resources related to red blood cell research. Whole red blood cells are available for complement assay and other studies. Purified hemoglobins as well as several heme-related porphyrin compounds are also available.
We offer glutaraldehyde-fixed sheep RBCs and antibody-sensitized sheep RBCs for complement assays. The antibody-sensitized sheep RBCs have a very short shelf-life and we recommend customers try to plan their assay schedules around our ship dates (approximately every three months).
Hemoglobin A, the most common form of hemoglobin, is a tetramer consisting of two 16 kDa α subunits and two structurally similar β subunits, with 141 and 146 amino acid residues, respectively.
Nomenclature and Conversion of Hemoglobin Forms
Oxidized State: Methemoglobin and Ferrihemoglobin are synonymous and refer to the oxidized +3 oxidation state of hemoglobin. Hemoglobin must be in the reduced form in order to bind oxygen.
Reduced State: Ferrous-hemoglobin: can exist as oxyhemoglobin or deoxyhemoglobin both of which are in the reduced +2 form. Oxyhemoglobin contains bound oxygen. Deoxyhemoglobin does not contain bound oxygen.
The following protocol is recommended and is based on the method of Dixon, H.B.F. and McIntosh, R. Nature, January 28, 213, 399-400, 1967.