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Merck

MAB374

Anti-GAPDH Antibody

CHEMICON®, mouse monoclonal, 6C5

Szinonimák:

glyceraldehyde 3-phosphate dehydrogenase, aging-associated gene 9 protein, glyceraldehyde-3-phosphate dehydrogenase, GAPDH, G3PDH, GAPD, OK/SW-cl.12, MGC88685, G3PD, EC 1.2.1.12

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez

Méret kiválasztása

Erről a tételről

UNSPSC kód:
12352203
eCl@ss:
32160702
NACRES:
NA.41

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Termék neve

Anti-Glyceraldehyde-3-Phosphate Dehydrogenase Antibody, clone 6C5, clone 6C5, Chemicon®, from mouse

biológiai forrás

mouse

Minőségi szint

100

antitest forma

purified immunoglobulin

antitest terméktípus

primary antibodies

klón

6C5, monoclonal

faj reaktivitás

human, feline, pig, mouse, rabbit, fish, canine, rat

nem léphet reakcióba

E. coli

gyártó/kereskedő neve

Chemicon®

technika/technikák

ELISA: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

izotípus

IgG1

NCBI elérési szám

NP_002037

UniProt elérési szám

P04406

kiszállítva

wet ice

célzott transzláció utáni módosítás

unmodified

Géninformáció

human ... GAPDH(2597)
mouse ... Gapdh(14433)

Kapcsolódó kategóriák

Általános leírás

38 kDa
Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is an ubiquitous glycolytic enzyme present in reasonably high levels in almost all tissues. As a ′house-keeping′ enzyme, it catalyzes the synthesis of 1,3-biphosphoglycerate, a "high energy" intermediate used for the synthesis of ATP. Besides its cytoplasmic action in metabolism it is also involved in the initial stages of apoptosis or oxidative stress response where GAPDH is translocated to the nucleus. Such actions may reflect the role of GAPDH in DNA repair or as one nuclear carrier for apoptotic molecules. GAPDH has also been found to bind specifically to proteins implicated in the pathogenesis of a variety of neurodegenerative disorders including the beta-amyloid precursor protein and the huntingtin protein where decreased function of GAPDH is associated with Alzheimer′s and Huntington′s disease fibroblasts. GAPDH has also been identified as a potential target for nitric oxide (NO)-mediated cellular toxicity. The complete and functional enzyme is a tetramer with each of four identical subunits occupying the vertex of a tetrahedron. Binding domains also include one principally interacting with NAD+ and another interacting with glyceraldehyde 3-phosphate (GAP).

Immunogén

Glyceraldehyde-3-phosphate dehydrogenase from rabbit muscle.

Alkalmazás

Anti-Glyceraldehyde-3-Phosphate Dehydrogenase Antibody, clone 6C5 is a well published and extensively characterized monoclonal antibody. This purified mAb detects Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) & has been published & validated for use in ELISA, IP, IC, IF, IH & WB.
ELISA:
1:1,000,000 dilution of a previous lot ws used for Immunoaffinity purification of GAPDH.

Immunocytochemistry:
1:100 to 1:300 dilution of a previous lot was used in immunocytochemistry in PBS-BSA 10 mg/mL. 4% PFA, with 0.02% PBS-Triton X-100; 3 min RT (Griffoni, 2001).

Western blot:
1:100 to 1:300. Recognizes a 36kDa band of the reduced monomer. Non-reduced GAPDH runs as a 146kDa tetramer.

Immunoaffinity purification of GAPDH.

Optimal working dilutions must be determined by end user.
Research Category
Metabolism
Research Sub Category
Enzymes & Biochemistry

Biokémiai/fiziológiai hatások

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from skeletal muscle. Antibody also recognizes cardiac GAPDH. GAPDH enzyme is detected in many non-muscle cells lines including HeLa, HCT-116 cells, U937 and THP-1 cells among others.
Human, porcine, canine, rat, mouse, rabbit, cat, and fish skeletal muscle. It has been reported that this antibody does not react with GAPDH from E. coli.

Fizikai forma

Format: Purified
Protein A purified
Purified Mouse monoclonal IgG1 in PBS, pH 7.4 with 0.1% sodium azide.

Elkészítési megjegyzés

Maintain at 2-8ºC in undiluted aliquots for up to 6 months after date of receipt.

Analízis megjegyzés

Control
It is expressed in all cells.
Evaluated by Western Blot on A431 lysates.

Western Blot Analysis:
1:500 dilution of this antibody detected GLYCERALDEHYDE-3-PDH on 10 μg of A431 lysates.

Egyéb megjegyzések

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Jogi információk

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Jogi nyilatkozat

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Tárolási osztály kódja

12 - Non Combustible Liquids

WGK

WGK 2

Lobbanási pont (F)

Not applicable

Lobbanási pont (C)

Not applicable

Analitikai tanúsítványok (COA)

Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.

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American Journal of Physiology: Renal Physiology null
An integrated systems analysis implicates EGR1 downregulation in simian immunodeficiency virus encephalitis-induced neural dysfunction.
Gersten, M; Alirezaei, M; Marcondes, MC; Flynn, C; Ravasi, T; Ideker, T; Fox, HS
The Journal of Neuroscience null
Studying protein degradation pathways in vivo using a cranial window-based approach.
Unni, VK; Ebrahimi-Fakhari, D; Vanderburg, CR; McLean, PJ; Hyman, BT
Methods null
Kristina Leuner et al.
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