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Méret kiválasztása
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UNSPSC Code:
12352200
Műszaki ügyfélszolgálat
Segítségre van szüksége? Szakértő tudósaink csapata készséggel áll az Ön rendelkezésére.
Segíthetünk?Műszaki ügyfélszolgálat
Segítségre van szüksége? Szakértő tudósaink csapata készséggel áll az Ön rendelkezésére.
Segíthetünk?usage
sufficient for 20 reactions
packaging
kit of 1
manufacturer/tradename
Roche
technique(s)
immunoprecipitation (IP): suitable
shipped in
wet ice
storage temp.
2-8°C
General description
Kit contains all reagents necessary for cell lysis, solubilization, stabilization, and immunopurification of proteins.
Application
The immunoprecipitation kit has been used for the immunoprecipitation of proteins from cellular extracts with Protein G Agarose.
Biochem/physiol Actions
Protein G is a cell wall protein, isolated from a specific bacterial strain, which has specific binding sites for certain classes of immunoglobulins from different species. Protein G binds nearly all subclasses of IgG, but no other classes of immunoglobulins.
Packaging
1 kit containing 5 components.
Preparation Note
Working solution: Lysis buffer/wash buffer 1
The kit contains reagents for 125 ml of lysis buffer/wash buffer 1. Prepare at least a minimal volume of 25 ml, sufficient for four immunoprecipitations.
To prepare 25 ml of lysis buffer/wash buffer 1 mix 5 ml core buffer, 3.75 ml NaCl, 2.5 ml detergent mix and 1 cOmplete Tablet. Add water to a final volume of 25 ml.
Solution is stable at 2 to 8 °C for 24 hours. When stored in aliquots at -15 to -25 °C, the solution is stable for at least four weeks. Mix thoroughly after thawing.
Wash buffer 2
The kit contains reagents for 50 ml of wash buffer 2. 2 ml of this buffer is required for one immunoprecipitation.
To prepare 50 ml of wash buffer 2 mix 10 ml core buffer, 25 ml NaCl and 0.5 ml detergent mix. Add water to a final volume of 50 ml.
Solution is stable at 2 to 8 °C. For longer periods, store aliquots at -15 to -25 °C. Mix thoroughly after thawing.
Wash buffer 3
The kit contains reagents for 25 ml of wash buffer 3. 1 ml of this buffer is required for one immunoprecipitation.
To prepare 25 ml mix 1 ml core buffer and 0.25 ml detergent mix. Add water to a final volume of 25 ml.
Solution is stable at 2 to 8 °C. For longer periods, store aliquots at -15 to -25 °C. Mix thoroughly after thawing.
The kit contains reagents for 125 ml of lysis buffer/wash buffer 1. Prepare at least a minimal volume of 25 ml, sufficient for four immunoprecipitations.
To prepare 25 ml of lysis buffer/wash buffer 1 mix 5 ml core buffer, 3.75 ml NaCl, 2.5 ml detergent mix and 1 cOmplete Tablet. Add water to a final volume of 25 ml.
Solution is stable at 2 to 8 °C for 24 hours. When stored in aliquots at -15 to -25 °C, the solution is stable for at least four weeks. Mix thoroughly after thawing.
Wash buffer 2
The kit contains reagents for 50 ml of wash buffer 2. 2 ml of this buffer is required for one immunoprecipitation.
To prepare 50 ml of wash buffer 2 mix 10 ml core buffer, 25 ml NaCl and 0.5 ml detergent mix. Add water to a final volume of 50 ml.
Solution is stable at 2 to 8 °C. For longer periods, store aliquots at -15 to -25 °C. Mix thoroughly after thawing.
Wash buffer 3
The kit contains reagents for 25 ml of wash buffer 3. 1 ml of this buffer is required for one immunoprecipitation.
To prepare 25 ml mix 1 ml core buffer and 0.25 ml detergent mix. Add water to a final volume of 25 ml.
Solution is stable at 2 to 8 °C. For longer periods, store aliquots at -15 to -25 °C. Mix thoroughly after thawing.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Csak a készletek alkatrészei
Termékszám
Leírás
- Core Buffer
- NaCl
- Detergent Mix
- cOmplete Protease Inhibitor Cocktail Tablets (5)
- Protein G Agarose ready-to-use
signalword
Danger
hcodes
Hazard Classifications
Aquatic Chronic 2 - Eye Dam. 1 - Skin Corr. 1B - Skin Sens. 1
Tárolási osztály
8B - Non-combustible corrosive hazardous materials
wgk
WGK 3
flash_point_f
does not flash
flash_point_c
does not flash
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Protokollok
Immunoprecipitation Kit (Protein G) Protocol & Troubleshooting
Ez a protokoll részletezi az immunprecipitációt immobilizált G-protein felhasználásával szövetekkel vagy sejtekkel. Hibaelhárítási tippeket is tartalmaz.
Kapcsolódó tartalom
Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.
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