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B2261
bisBenzimide H 33342 trihydrochloride
≥98% purity (HPLC and TLC), powder
Szinonimák:
2′-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5′-bi-1H-benzimidazole trihydrochloride, HOE 33342, Hoechst 33342, bisBenzimide
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Méret kiválasztása
25 MG
17 100,00 Ft
100 MG
46 700,00 Ft
500 MG
187 000,00 Ft
1 G
335 000,00 Ft
17 100,00 Ft
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Erről a tételről
Tapasztalati képlet (Hill-képlet):
C27H28N6O · 3HCl · xH2O
CAS-szám:
Molekulatömeg:
561.93 (anhydrous basis)
NACRES:
NA.47
PubChem Substance ID:
UNSPSC Code:
12171500
MDL number:
Beilstein/REAXYS Number:
1234011
Ugrás ide:
Műszaki ügyfélszolgálat
Segítségre van szüksége? Szakértő tudósaink csapata készséggel áll az Ön rendelkezésére.
Segíthetünk?Termék neve
bisBenzimide H 33342 trihydrochloride, ≥98% (HPLC)
InChI
1S/C29H32N6O.3ClH/c1-3-36-25-8-4-20(5-9-25)28-30-18-22-16-21(6-10-26(22)32-28)29-31-19-23-17-24(7-11-27(23)33-29)35-14-12-34(2)13-15-35;;;/h4-11,16-17H,3,12-15,18-19H2,1-2H3,(H,30,32)(H,31,33);3*1H
SMILES string
Cl[H].Cl[H].Cl[H].CCOc1ccc(cc1)C2=NCc3cc(ccc3N2)C4=NCc5cc(ccc5N4)N6CCN(C)CC6
InChI key
FYEVKHPLBHLWHK-UHFFFAOYSA-N
form
powder
technique(s)
titration: suitable
color
yellow
pH
1.7 (20 °C)
solubility
H2O: 20 mg/mL, phosphate buffer: precipitates
suitability
suitable for fluorescence
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
−20°C
Quality Level
assay
≥98% (HPLC)
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Kapcsolódó kategóriák
1 of 4
Ez a tétel | |||
|---|---|---|---|
| technique(s) titration: suitable | technique(s) - | technique(s) - | technique(s) - |
| suitability suitable for fluorescence | suitability - | suitability - | suitability - |
| color yellow | color - | color - | color - |
| form powder | form - | form powder | form powder |
| solubility H2O: 20 mg/mL, phosphate buffer: precipitates | solubility - | solubility H2O: 10 mg/mL, water and ethanol: 10 mg/mL, phosphate buffer: precipitates | solubility water and ethanol: 10 mg/mL, H2O: soluble, ethanol: soluble |
| assay ≥98% (HPLC and TLC) | assay ≥97.0% (HPLC) | assay ≥98% (HPLC and TLC) | assay ≥98.0% (HPLC) |
General description
Application
Bisbenzimide Hoechst 33342 is a specific stain for AT-rich regions of double-stranded DNA and has been shown to displace several known DNA intercalators.[2] This fluorescent dye has been used in sorting living cells based on DNA content,[3] used in flow cytometry for the determination of DNA content, and for the visualization of chromatin distribution in living cells.[4] It has been used to detect BrdU incorporation into cells[5] and in studying the initial stages apoptosis and cellcycle distribution.[6],[7] Chromosomes that are dividing or replicating will not stain with this dye.
Useful for staining DNA, chromosomes and nuclei. May be used for fluorescence microscopy or flow cytometry.
Excitation max. = 346 nm
Emission max. = 460 nm
Excitation max. = 346 nm
Emission max. = 460 nm
Biochem/physiol Actions
Membrane-permeable, fluorescent DNA stains with low cytotoxicity that intercalate in A-T regions of DNA.
Preparation Note
This product is soluble in water (50 mg/ml), yielding a clear solution. The pH of a 2% solution is 1.9. It has been observed that this material will precipitate from phosphate buffer solutions.
Aqueous solutions are stable for 1 month if kept in the dark at 2-8 °C.[4]
Aqueous solutions are stable for 1 month if kept in the dark at 2-8 °C.[4]
related product
Termékszám
Leírás
Árazás
Tárolási osztály
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
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Már rendelkezik ezzel a termékkel?
Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.
D J Arndt-Jovin et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 25(7), 585-589 (1977-07-01)
The methods for measuring the deoxyribonucleic acid content of individual mammalian cells and sorting them on the basis of this parameter have until now required fixation or other treatment which renders the cells nonviable. Using a class of bis-benzimidazole dyes
P E Mozdziak et al.
Cytometry, 41(2), 89-95 (2000-09-26)
5-Bromo-2'-deoxyuridine (BrdU) is a powerful compound to study the mitotic activity of a cell. Most techniques that identify BrdU-labeled cells require conditions that kill the cells. However, the fluorescence intensity of the membrane-permeable Hoechst dyes is reduced by the incorporation
Basic helix-loop-helix transcriptional factor MyoR regulates BMP-7 in acute kidney injury.
Kamiura N, et al.
American Journal of Physiology: Renal Physiology, 304, F1159-F1166 (2013)
M G Ormerod et al.
Cytometry, 14(6), 595-602 (1993-01-01)
We have recently developed a method for the separation and quantification of viable apoptotic cells without the need for permeabilisation or fixation of the cells. The method is based on the observation that apoptotic rat thymocytes fluoresce more brightly than
F Belloc et al.
Cytometry, 17(1), 59-65 (1994-09-01)
A flow cytometric method to detect apoptotic cells is described. This method is based on the detection of differences in chromatin condensation with Hoechst 33342 as a probe and the detection of dead cells with propidium iodide as a probe
Cikkek
Cell cycle regulates vital processes like DNA repair, cancer prevention. Four stages: G1, S, G2, M. NTPs don't permeate membranes.
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What is the Department of Transportation shipping information for this product?
1 answer-
Helpful?
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Which Hoechst dye is suitable for chromosomal analysis?
1 answer-
Hoechst 33258 (Product No. B2883) is the preferred Hoechst dye for chromosomal analysis. Hoechst 33342 (Product No. B2261) is a poor fluorochrome for chromosomal analysis.
Helpful?
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Can solvents other than water be used to dissolve Product B2261, bisBenzimide H 33342 trihydrochloride?
1 answer-
Hoechst 33342 can be dissolved in N,N-dimethylformamide (DMF).
Helpful?
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What is a typical concentration of Product B2261, bisBenzimide H 33342 trihydrochloride, for staining DNA in cells?
1 answer-
200 micrograms per mL.
Helpful?
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