Yes, this product is stable until recommended retest date in original container. This product is a prepared solution of 1M DTT in water. In the original sealed container it is quite stable, with an assigned retest schedule of every 2 years. Assuming that the product is handled properly, the material is expected to be stable until at least the retest date reported on the lot specific Certificate of Analysis.
43816
DL-Dithiothreitol solution
BioUltra, Molecular Biology, ~1 M in H2O
Szinonimák:
Cleland′s reagent, (±)-Dithiothreitol, rac-Dithiothreitol, Dithiothreitol, (±)-threo-1,4-Dimercapto-2,3-butanediol solution, DTT
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Méret kiválasztása
10 ML
12 500,00 Ft
50 ML
42 100,00 Ft
250 ML
142 000,00 Ft
12 500,00 Ft
Az elérhetőséggel kapcsolatos kérdésekkel kérjük, forduljon Vevőszolgálatunkhoz.
Erről a tételről
Tapasztalati képlet (Hill-képlet):
C4H10O2S2
CAS-szám:
Molekulatömeg:
154.25
Beilstein:
1719757
MDL-szám:
UNSPSC kód:
12352201
PubChem Substance ID:
NACRES:
NA.25
Ugrás ide:
Műszaki ügyfélszolgálat
Segítségre van szüksége? Szakértő tudósaink csapata készséggel áll az Ön rendelkezésére.
Segíthetünk?grade
Molecular Biology
Minőségi szint
termékcsalád
BioUltra
Teszt
900-1100 mmol/L (Titration molarity)
Forma
liquid
reakcióalkalmasság
reagent type: reductant
koncentráció
~1 M in H2O
szennyeződések
DNases, none detected
RNases, none detected
phosphatases, none detected
proteases, none detected
pH
2.7-5.0
sűrűség
1.04 g/mL at 20 °C
Abszorpció
cut-off at 330 nm
alkalmasság
in accordance for filter test
suitable for molecular biology
tárolási hőmérséklet
2-8°C
SMILES string
O[C@H](CS)[C@H](O)CS
InChI
1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m1/s1
Nemzetközi kémiai azonosító kulcs
VHJLVAABSRFDPM-QWWZWVQMSA-N
Hasonló termékeket keres? Látogasson el ide Útmutató a termékösszehasonlításhoz
1 of 4
Ez a tétel | 646563 | D0632 | D9163 |
|---|---|---|---|
| grade for molecular biology | grade - | grade - | grade - |
| assay 900-1100 mmol/L (Titration molarity) | assay - | assay ≥98% (HPLC), ≥99.0% (titration) | assay ≥99% |
| Quality Level 200 | Quality Level 100 | Quality Level 200 | Quality Level 200 |
| product line BioUltra | product line - | product line - | product line - |
| storage temp. 2-8°C | storage temp. - | storage temp. 2-8°C | storage temp. 2-8°C |
| impurities DNases, none detected, phosphatases, none detected, RNases, none detected, proteases, none detected | impurities - | impurities - | impurities - |
Általános leírás
Dithiothreitol (DTT), a thiol group protectant,[1] is an excellent reagent for preventing the oxidation of SH groups in proteins. DTT solution is a potent and versatile disulfide-reducing agent.[2] It reduces a disulfide bond by forming a six-membrane ring and initiating a thiol-disulfide interchange.
Alkalmazás
DL-dithiothreitol solution has been used:
- in the treatment of intestinal explants[3]
- in homogenate preparation of gill cells for enzymatic assays[4]
- in protein electrophoresis for western blotting[5]
- in transglutaminase activity[6]
- in sample buffer for SDS-PAGE[7]
- in Western Blot, as a lysis buffer component for single-nucleus RNA-sequencing[8]
- as a component of protein extraction buffer for cell lysis[8]
Biokémiai/fiziológiai hatások
DL-dithiothreitol (DTT) is used in a chemical debonding technique, that helps to remove bacteria from biofilms.[9] It can change the structural stability of the GABA(B) (gamma-aminobutyric acid) receptor by disabling the disulfide bonds between four cysteine residues positioned in the GABA(B1(a)) receptor structure.[10]Dithiothreitol (DTT) is broadly utilized in synthetic peptide synthesis and biochemical arrangements of thiol proteins. It can also be used to study protein chemistry topics like enzyme activity and protein folding. DTT explicitly controls the thiol-disulfide exchange response to completely reduce the intra-or between sub-atomic disulfide bonds in biomolecules. Thiols and the cyclic disulfide of DTT are the products of this reaction.
Tulajdonságok és előnyök
- BioUltra grade - 1M DTT solution, suitable for molecular biology
- Free from DNases, RNases, phosphatases, and proteases
Egyéb megjegyzések
For additional information on our range of Biochemicals, please complete this form.
kapcsolódó termék
Termékszám
Leírás
Árazás
összehasonlítható termékek
Termékszám
Leírás
Árazás
Figyelmeztetés
Danger
Figyelmeztető mondatok
Óvintézkedésre vonatkozó mondatok
Veszélyességi osztályok
Eye Dam. 1 - Skin Irrit. 2
Tárolási osztály kódja
10 - Combustible liquids
WGK
WGK 3
Lobbanási pont (F)
Not applicable
Lobbanási pont (C)
Not applicable
Egyéni védőeszköz
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
Válasszon a legfrissebb verziók közül:
Már rendelkezik ezzel a termékkel?
Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.
Expression and Localization of Cathepsins B, D, and G in Dupuytren?s Disease
Tan K, et al.
Plastic and reconstructive surgery. Global open, 6(2), e0133549-e0133549 (2018)
New Technologies, An Issue of Orthopedic Clinics (2018)
Suppression of GABA B receptor function in vivo by disulfide reducing agent, dl-dithiothreitol (DTT)
Carai M A M, et al.
Psychopharmacology, 174(2), 283-290 (2004)
In Utero Exposure to Maternal Diabetes Is Associated With Early Abnormal Vascular Structure in Offspring
Dib A, et al.
Frontiers in physiology, 9, 350-350 (2018)
Delivery of human immunodeficiency virus vaccine vectors to the intestine induces enhanced mucosal cellular immunity
Wang L, et al.
Journal of Virology, 83(14), 7166-7175 (2009)
Cikkek
Bis-Tris SDS-PAGE gel casting kits with mPAGE® TurboMix technology for rapid, reliable gel casting and protein separation.
Protokollok
Simplify your SDS-PAGE gel preparation with our pre-mixed solutions and protocols to achieve precise protein separation.
Egyszerűsítse az SDS-PAGE gél előkészítését előre kevert oldatainkkal és protokolljainkkal a fehérjék precíz elválasztása érdekében.
-
After opening DTT ref 43816-50ML, is the product stable? Can it be reused?
1 answer-
Helpful?
-
-
How can I determine the shelf life / expiration / retest date of this product?
1 answer-
If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdfHelpful?
-
-
How is shipping temperature determined? And how is it related to the product storage temperature?
1 answer-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
Helpful?
-
-
How stable is this DTT solution ? it is known that DTT is unstable in solution and should be kept under inert atmosphere. How long can I keep it once open ?
1 answer-
This product is a prepared solution of 1M DTT in water. In the original sealed container it is quite stable, with an assigned retest schedule of every 2 years. Assuming that the product is handled properly, the material is expected to be stable until at least the retest date reported on the lot specific Certificate of Analysis.
Helpful?
-
Active Filters
Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.
Lépjen kapcsolatba a szaktanácsadással