C9584

Carboxypeptidase B from porcine pancreas

lyophilized powder

• Szinonimák: Peptidyl-L-lysine(L-arginine) hydrolase, Protaminase
• CAS-szám: 9025-24-5
• PubChem Substance ID: 24893175
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 232-788-9
• MDL number: MFCD00081476
• EC-szám: 3.4.17.2 (BRENDA, IUBMB)

Tulajdonságok

• Termék neve: Carboxypeptidase B from porcine pancreas, lyophilized powder
• SMILES string: S(Cc3ccccc3)C(=O)C(NC(=O)C2N(CCC2)C(=O)C(NC(=O)C(NC(=O)CCC(=O)O)C)C)Cc1ccccc1
• InChI key: TWURVFFNODFJBJ-UHFFFAOYSA-N
• InChI: 1S/C31H38N4O7S/c1-20(32-26(36)15-16-27(37)38)28(39)33-21(2)30(41)35-17-9-14-25(35)29(40)34-24(18-22-10-5-3-6-11-22)31(42)43-19-23-12-7-4-8-13-23/h3-8,10-13,20-21,24-25H,9,14-19H2,1-2H3,(H,32,36)(H,33,39)(H,34,40)(H,37,38)
• biological source: Porcine pancreas
• grade: Proteomics Grade
• form: lyophilized powder
• specific activity: ≥125 units/mg protein
• mol wt: 34,000 Da± 600
• purified by: affinity chromatography
• composition: Protein, 40-70%
• foreign activity: carboxypeptidase A ≤1%; chymotrypsin ≤0.1%; trypsin ≤5%
• storage temp.: −20°C
• Quality Level: 200

Leírás

Analysis Note

Protein determined by biuret.

Application

Carboxypeptidase B has been used in a study to develop a non-invasive pregnancy assay for use in both captive and wild polar bears. Carboxypeptidase B has been used in a study that identified new potential biomarkers of acute pancreatitis.

The enzyme from Sigma has been used to develop homogeneous time-resolved fluorescence (HTRF) assay for measuring carboxypeptidase B activity in a miniaturized high-throughput screening format. It has been used to evaluate the impact of the C-terminal lysine(s) in human plasminogen binding to Bifidobacterium. The effect of treatment with carboxypeptidase B, which is a C-terminal lysine-specific endopeptidase, is measured using flow cytometry analysis.

Biochem/physiol Actions

Carboxypeptidase B is a proteolytic enzyme capable of rapidly hydrolyzing peptide bonds to release certain carboxyl-terminal basic amino acids from peptides and proteins. Its molecular mass is 34,300±600 Da. It contains one non-dialyzable gram atom of zinc per mole. The enzyme activity is inhibited by metal chelating agents 1, 10-phenanthroline, 8-hydroxyquinoline-5-sulfonic acid, and 2,2′-dipyridyl.

Other Notes

One unit will hydrolyze 1.0 μmole of hippuryl-L-arginine per min at pH 7.65 at 25 °C.

Packaging

Package size based on protein content

Physical form

Contains HEPES buffer salts, zinc chloride and carbohydrate

Preparation Note

Treated with protease inhibitor, AEBSF, to eliminate serine protease activity.

Biztonsági adatok

• pictograms: GHS08,GHS07
• signalword: Danger
• hcodes: H315,H319,H334,H335
• pcodes: P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• target_organs: Respiratory system
• Tárolási osztály: 10 - Combustible liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves