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P7255

Sigma-Aldrich

Puromycin dihydrochloride from Streptomyces alboniger

greener alternative

≥98% (HPLC), powder

Szinonimák:

3′-[α-Amino-p-methoxyhydrocinnamamido]-3′-deoxy-N,N-dimethyladenosine dihydrochloride

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez

Méret kiválasztása

25 MG
27 700,00 Ft
100 MG
83 900,00 Ft
250 MG
183 000,00 Ft
500 MG
371 000,00 Ft
1 G
629 000,00 Ft

27 700,00 Ft

Az elérhetőséggel kapcsolatos kérdésekkel kérjük, forduljon Vevőszolgálatunkhoz.
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Összes fotó(10)

Méret kiválasztása

Nézet módosítása
25 MG
27 700,00 Ft
100 MG
83 900,00 Ft
250 MG
183 000,00 Ft
500 MG
371 000,00 Ft
1 G
629 000,00 Ft

About This Item

Tapasztalati képlet (Hill-képlet):
C22H29N7O5 · 2HCl
CAS-szám:
58-58-2
Molekulatömeg:
544.43
Beilstein:
3853613
EC-szám:
200-387-8
MDL-szám:
MFCD00012691
UNSPSC kód:
51281912
PubChem Substance ID:
57654556
NACRES:
NA.77

27 700,00 Ft

Az elérhetőséggel kapcsolatos kérdésekkel kérjük, forduljon Vevőszolgálatunkhoz.
Request a Bulk Order

Minőségi szint

300

Teszt

≥98% (HPLC)

Forma

powder

környezetbarátabb alternatív termék pontszám

old score: 88
new score: 79
Find out more about DOZN™ Scoring

környezetbarátabb alternatív termék tulajdonságai

Atom Economy
Design for Energy Efficiency
Use of Renewable Feedstocks
Design for Degradation
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

oldhatóság

H2O: 50 mg/mL

antibiotikus hatásspektrum

Gram-positive bacteria
neoplastics
parasites

környezetbarátabb alternatív kategória

, Re-engineered

Hatásmechanizmus

protein synthesis | interferes

tárolási hőmérséklet

−20°C

SMILES string

Cl.Cl.COc1ccc(C[C@H](N)C(=O)N[C@H]2[C@@H](O)[C@@H](O[C@@H]2CO)n3cnc4c(ncnc34)N(C)C)cc1

InChI

1S/C22H29N7O5.2ClH/c1-28(2)19-17-20(25-10-24-19)29(11-26-17)22-18(31)16(15(9-30)34-22)27-21(32)14(23)8-12-4-6-13(33-3)7-5-12;;/h4-7,10-11,14-16,18,22,30-31H,8-9,23H2,1-3H3,(H,27,32);2*1H/t14-,15+,16+,18+,22+;;/m0../s1

Nemzetközi kémiai azonosító kulcs

MKSVFGKWZLUTTO-FZFAUISWSA-N

Hasonló termékeket keres? Látogasson el ide Útmutató a termékösszehasonlításhoz

Kapcsolódó kategóriák

Általános leírás

Chemical structure: peptidyl nucleoside
Sterilize stock solution by filtration using 0.22 μm filter then store in aliquots at −20 °C.
We are committed to bringing you Greener Alternative Products, which adhere to one of the four categories of Greener Alternatives . This product belongs to category of Re-engineered products, showing key improvements in Green Chemistry Principles “Atom Economy”, “Design for Energy Efficiency”, “Use of Renewable Feedstock” and “Design for Degradation”. Click here to view its DOZN scorecard.

Alkalmazás

Recommended for use as a selection agent at a range of 1-10 μg/mL.

Biokémiai/fiziológiai hatások

Mode of Action: Puromycin inhibits protein synthesis by causing premature chain termination acting as an analog of the 3′-terminal end of the aminoacyl-tRNA.

Mode of Resistance: Puromycin acetyltransferase is an effective resistance gene.

Antimicrobial Spectrum: This product is active against gram-positive microorganisms, less active against acid-fast bacilli and more weakly active against gram-negative microorganisms. Puromycin can prevent growth of bacteria, protozoa, algae and mammalian cells and acts quickly, killing 99% of cells within 2 days.
Puromycin dihydrochloride is a part of the amino-nucleoside family of antibiotics and is derived from Streptomyces alboniger. It is a broad spectrum antibiotic with antitumor activity, as an inhibitor of protein synthesis and has been used to study transcription regulatory mechanisms that control the sequential and coordinate expression of genes during cell differentiation. The recommended working concentration in eukaryotic cell culture is 1-10 μg/mL.

Tulajdonságok és előnyök

This compound is a featured product for Gene Regulation research. Click here to discover more featured Gene Regulation products. Learn more about bioactive small molecules for other areas of research at sigma.com/discover-bsm.

Vigyázat

As supplied, this product remains active for four years when stored at -20°C.

Elkészítési megjegyzés

This product is soluble in water at 50 mg/mL. A stock solution should be prepared by filtration using a 0.22 μm filter and then stored in aliquots at -20°C. This product is also soluble in methanol at 10 mg/mL.

Egyéb megjegyzések

Keep container tightly closed in a dry and well-ventilated place.Keep in a dry place. Keep in a dry place

Piktogramok

Exclamation mark
GHS07

Figyelmeztetés

Warning

Figyelmeztető mondatok

H302

Óvintézkedésre vonatkozó mondatok

P301 + P312 + P330

Veszélyességi osztályok

Acute Tox. 4 Oral

Tárolási osztály kódja

11 - Combustible Solids

WGK

WGK 3

Lobbanási pont (F)

Not applicable

Lobbanási pont (C)

Not applicable

Egyéni védőeszköz

dust mask type N95 (US), Eyeshields, Gloves

Válasszon a legfrissebb verziók közül:

Analitikai tanúsítványok (COA)

Lot/Batch Number
0000436145
0000436145
0000422730
0000422730
0000422728

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Ha egy adott verzióra van szüksége, a tétel- vagy cikkszám alapján rákereshet egy adott tanúsítványra.

COA keresése

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Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.

Dokumentumtár megtekintése

Robert A J Signer et al.
Nature, 509(7498), 49-54 (2014-03-29)
Many aspects of cellular physiology remain unstudied in somatic stem cells, for example, there are almost no data on protein synthesis in any somatic stem cell. Here we set out to compare protein synthesis in haematopoietic stem cells (HSCs) and
Shingo Ueno et al.
Journal of biotechnology, 162(2-3), 299-302 (2012-09-25)
cDNA display using a puromycin-linker to covalently bridge a protein and its coding cDNA is a stable and efficient in vitro protein selection method. The optimal design of the often-used puromycin-linker is vital for effective selection. In this report, an
Antonina J Kruppa et al.
Biochimica et biophysica acta, 1832(12), 2115-2126 (2013-08-06)
The accumulation of β-amyloid (Aβ) peptide in the brain is one of the pathological hallmarks of Alzheimer's disease and is thought to be of primary aetiological significance. In an unbiased genetic screen, we identified puromycin-sensitive aminopeptidase (PSA) as a potent
Claudia Fritsch et al.
Genome research, 22(11), 2208-2218 (2012-08-11)
So far, the annotation of translation initiation sites (TISs) has been based mostly upon bioinformatics rather than experimental evidence. We adapted ribosomal footprinting to puromycin-treated cells to generate a transcriptome-wide map of TISs in a human monocytic cell line. A
Robert J Huber et al.
Biochimica et biophysica acta, 1833(1), 11-20 (2012-10-16)
Cyclin-dependent kinase 5 (Cdk5) is a serine/threonine kinase that has been implicated in a number of cellular processes. In Dictyostelium, Cdk5 localizes to the nucleus and cytoplasm, interacts with puromycin-sensitive aminopeptidase A (PsaA), and regulates endocytosis, secretion, growth, and multicellular
Összes kapcsolódó tanulmány megtekintése

Cikkek

Inhibition of Protein Synthesis by Antibiotics

Antibiotics targeting bacterial ribosomes disrupt protein synthesis, a key process in bacterial growth inhibition.

Discover Bioactive Small Molecules for Gene Regulation

We offer a variety of small molecule research tools, such as transcription factor modulators, inhibitors of chromatin modifying enzymes, and agonists/antagonists for target identification and validation in gene regulation research; a selection of these research tools is shown below.

Questions

1–5 of 5 Questions  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  3. What are the approximate concentrations of Product P7288, Puromycin dihydrochloride, suitable for HEK293, A431 and Jurkat cells?

    1 answer

    1. HEK293: 2 μg/mL; A431: 1 μg/mL and Jurkat: 4 μg/mL.

      Helpful?

  4. What are the approximate concentrations of Product P7255, Puromycin dihydrochloride, suitable for HeLa human uterus, B16 mouse melanoma and PC1.0 hamster adenocarcinoma cells?

    1 answer

    1. HeLa human uterus: 3 μg/mL; B16 mouse melanoma: 1-3 μg/mL; PC1.0 hamster adenocarcinoma: 10 μg/mL

      Helpful?

  5. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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