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UPS2

Proteomics Dynamic Range Standard Set

Protein Mass Spectrometry Calibration Standard

Szinonimák:

Dynamic Range Standards, Proteomics Standard Set

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Méret kiválasztása


Erről a tételről

UNSPSC Code:
12161503
EC Number:
232-650-8
NACRES:
NA.24
Form:
ready-to-use solution
Biological source:
human
Műszaki ügyfélszolgálat
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Segítségre van szüksége? Szakértő tudósaink csapata készséggel áll az Ön rendelkezésére.
Segíthetünk?

Termék neve

Proteomics Dynamic Range Standard Set, Protein Mass Spectrometry Calibration Standard

biological source

human

form

ready-to-use solution

quality

Protein Mass Spectrometry Calibration Standard

concentration

10.6 μg/ampule protein

technique(s)

mass spectrometry (MS): suitable

shipped in

wet ice

storage temp.

−20°C

Quality Level

Application

Proteomics Dynamic Range Standard Set has been used in the intensity-based absolute quantification (iBAQ) of E .coli proteins, embryonic stem cells (ESCs) and neuronal precursor cells (NPCs) proteomes. It has also been used as a standard to spike HeLa cells for label-free quantification.
The Proteomics Dynamic Range Standard Set can be used to standardize and/or evaluate mass spectrometric (e.g., LC-MS/MS, MALDI-TOF-MS, etc.) and electrophoretic analysis conditions prior to the analysis of complex protein samples. UPS2 can be used to bracket precious experimental data sets between runs of a known complex standard sample. This allows confirmation of the robustness of the analysis method and stability of the instrument employed. Additionally, laboratories generating or comparing mass spectrometric data derived from poorly defined samples can use UPS2 as an external reference to assist with the evaluation of results and experimental methodology.
Proteomics Dynamic Range Standard Set has been used for the quantification of dynamic range universal protein standard on Orbitrap Analyzer using all ion fragmentation. It has been used as a standard for intensity-based absolute quantification of proteins (iBAQ) in LC-MS (liquid chromatography-mass spectrometry)/MS analysis.

General description

The Proteomics Dynamic Range Standard Set is produced from a mixture of 48 individual human source or human sequence recombinant proteins, each of which has been selected to limit heterogeneous post-translational modifications (PTMs). The protein standard is formulated from 6 mixtures of 8 proteins to present a dynamic range of 5 orders of magnitude, ranging from 50 pmoles to 500 amoles. Each protein has been quantitated by amino acid analysis (AAA) prior to formulation.

A készletek elemei külön is megvásárolhatók

Termékszám
Leírás
Biztonsági adatlap

  • T6567Trypsin from porcine pancreas, Proteomics Grade, BioReagent, Dimethylated 20 μgBiztonsági adatlap

signalword

Danger

Hazard Classifications

Eye Dam. 1 - Repr. 1B - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Tárolási osztály

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

flash_point_f

Not applicable

flash_point_c

Not applicable


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Analitikai tanúsítványok (COA)

Lot/Batch Number

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Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.

Dokumentumtár megtekintése

Erik Ahrné et al.
Proteomics, 13(17), 2567-2578 (2013-06-25)
There is a great interest in reliable ways to obtain absolute protein abundances at a proteome-wide scale. To this end, label-free LC-MS/MS quantification methods have been proposed where all identified proteins are assigned an estimated abundance. Several variants of this
An interaction landscape of ubiquitin signaling
Zhang X, et al.
Molecular Cell, 65(5), 941-955 (2017)
Boumediene Soufi et al.
Frontiers in microbiology, 6, 103-103 (2015-03-06)
We set out to provide a resource to the microbiology community especially with respect to systems biology based endeavors. To this end, we generated a comprehensive dataset monitoring the changes in protein expression, copy number, and post translational modifications in
Björn Schwanhäusser et al.
Nature, 473(7347), 337-342 (2011-05-20)
Gene expression is a multistep process that involves the transcription, translation and turnover of messenger RNAs and proteins. Although it is one of the most fundamental processes of life, the entire cascade has never been quantified on a genome-wide scale.
Andrew F Jarnuczak et al.
Journal of proteome research, 15(9), 2945-2959 (2016-07-28)
Quantitative mass spectrometry-based proteomics of complex biological samples remains challenging in part due to the variability and charge competition arising during electrospray ionization (ESI) of peptides and the subsequent transfer and detection of ions. These issues preclude direct quantification from

Cikkek

High-throughput proteomics advances with improved analysis methods and mass spectrometry.

Kapcsolódó tartalom

Standardize research with Universal and Dynamic Proteomics Standards, complex and well-characterized reference standards for mass spectrometry.

Instructions

Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.

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